|Table of Contents|

Targeted inhibition of triple-negative breast cancer by fusion protein anti-EGFR-iRGD modified extracellular vesicles of red blood cells combined with siRNA

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 17
Page:
3197-3205
Research Field:
Publishing date:

Info

Title:
Targeted inhibition of triple-negative breast cancer by fusion protein anti-EGFR-iRGD modified extracellular vesicles of red blood cells combined with siRNA
Author(s):
WEI Xueni; YANG Zhonghui; HUANG Ji; CHEN Guomei; CHENG Changjuan
Department of Pharmacy,Suzhou University Affiliated Taicang Hospital（the First People's Hospital of Taicang）,Jiangsu Taicang 215400,China.
Keywords:
red blood cell-derived extracellular vesicles; triple-negative breast cancer; siRNA; epithelial-mesenchymal transition; lipid insertion technique; Catenin Beta 1
PACS:
R737.9
DOI:
10.3969/j.issn.1672-4992.2024.17.006
Abstract:
Objective:To develop a targeted delivery system using red blood cell-derived extracellular vesicles（RBCEVs) to enhance the inhibitory efficiency against triple-negative breast cancer（TNBC).Methods:The fusion protein anti-epidermal growth factor receptor（EGFR)-iRGD peptide was incorporated into the membrane surface of RBCEVs using lipid insertion method to construct a biocompatible drug delivery system.Catenin Beta 1（CTNNB1) siRNA was effectively loaded onto anti-EGFR-iRGD-RBCEVs through electroporation.The inhibitory effects of anti-EGFR-iRGD-si-CTNNB1-RBCEVs on TNBC proliferation and migration were further examined,along with their regulation of epithelial-mesenchymal transition（EMT)-related protein expression.Results:The anti-EGFR-iRGD-si-CTNNB1-RBCEVs complex demonstrated high serum stability within 120 h and effectively prevented degradation of siRNA by RNAases.Moreover,this complex significantly enhanced the inhibitory effect of si-CTNNB1 on both the CTNNB1 gene and protein in TNBC cells,suppressing of TNBC proliferation and migration.The expression of Snail,Vimentin,and N-cadherin proteins was downregulated,while the expression of E-cadherin protein was promoted.Conclusion:This study demonstrates the targeted inhibition of TNBC cells using fusion protein anti-EGFR-iRGD modified RBCEVs in combination with siRNA.These findings provide a promising therapeutic approach for reating TNBC with high expression of integrin αvβ3.

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江苏省苏州市科技发展计划（民生科技-医疗卫生应用基础研究）项目（编号：SYSD2020172）
Last Update: 2024-07-31