|Table of Contents|

The effect of Zhuangxuan drink on autophagy and apoptosis of alveolar epithelial cells in H1N1 pneumonia mice based on SIRT1/AMPK signaling pathway

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 17
Page:
3190-3196
Research Field:
Publishing date:

Info

Title:
The effect of Zhuangxuan drink on autophagy and apoptosis of alveolar epithelial cells in H1N1 pneumonia mice based on SIRT1/AMPK signaling pathway
Author(s):
ZOU Min1; WEI Xing2; LAN Yuying2; ZHANG Yun2
1.Department of Pediatrics,Guangxi International Zhuang Medicine Hospital & Guangxi International Zhuang Medicine Hospital Affiliated to Guangxi University of Chinese Medicine,Guangxi Nanning 530200,China;2.School of Zhuang Medicine,Guangxi University of Chinese Medicine,Guangxi Nanning 530200,China.
Keywords:
Zhuangxuan drink; SIRT1/AMPK signaling pathway; pulmonary alveolar epithelial cells; autophagy; apoptosis
PACS:
R285.5；R363
DOI:
10.3969/j.issn.1672-4992.2024.17.005
Abstract:
Objective:To investigate the effect of Zhuangxuan drink on autophagy and apoptosis of alveolar epithelial cells in H1N1 pneumonia mice based on the silencing information regulator 1（SIRT1)/adenosine activated monophosphate protein kinase（AMPK) signaling pathway.Methods:Six mice were randomly selected as the control group,while the remaining mice were given 100 μL of influenza A virus（IAV) ［A/Puerto Rico/8/34（PR8,H1N1)］ virus solution through nasal drip to establish an H1N1 pneumonia mice model.Successfully modeled mice were randomly separated into H1N1 group,low-Zhuangxuan drink group（7.16 g·kg-1),medium-Zhuangxuan drink group（14.33 g·kg-1),high-Zhuangxuan drink group（28.66 g·kg-1),and high-Zhuangxuan drink+Compound C group（28.66 g·kg-1 Zhuangxuan drink+0.25 mg·kg-1 SIRT1/AMPK signaling pathway inhibitor Compound C),with 6 mice in each group,once in the morning and once in the afternoon,for 5 consecutive days.The H1N1 group and control group were given equal amounts of physiological saline.ELISA method was applied to detect the levels of inflammatory factors in BALF.HE staining was applied to detect pathological changes in lung tissue.The mouse alveolar epithelial cells were isolated and cultured.Flow cytometry was applied to detect apoptosis of alveolar epithelial cells.Western blot was applied to detect the expression of autophagy proteins and SIRT1/AMPK signaling pathway related proteins in alveolar epithelial cells.Results:The lung tissue structure in the control group was normal,while in the H1N1 group,inflammatory cells infiltrated into the interstitium,and severe alveolar bleeding,collapse,interstitial edema,and thickening of alveolar walls were observed in the lung tissue.Compared with the control group,the levels of interferon-γ（IFN-γ),interleukin-6（IL-6),tumor necrosis factor-α（TNF-α),and apoptosis rate of alveolar epithelial cells in the H1N1 group were obviously increased（P＜0.05),and the protein levels of AMPK,SIRT1,microtubule associated protein 1 light chain 3（LC3)-Ⅱ/Ⅰ,and benzyl chloride 1（Beclin 1) were significantly decreased（P＜0.05).Compared with the H1N1 group,the levels of IFN-γ,IL-6,TNF-α,and apoptosis rate of alveolar epithelial cells in the low-Zhuangxuan drink group,medium-Zhuangxuan drink group,and high-Zhuangxuan drink group were obviously reduced（P＜0.05),and the protein levels of AMPK,SIRT1,LC3-Ⅱ/Ⅰ,and Beclin 1 were obviously increased（P＜0.05),and there was a dose correlation.Compound C reversed the beneficial effects of high-Zhuangxuan drink.Conclusion:Zhuangxuan drink may improve autophagy and apoptosis of alveolar epithelial cells in H1N1 pneumonia mice by regulating the SIRT1/AMPK signaling pathway.

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Last Update: 2024-07-31