|Table of Contents|

Effect of YTHDF3 targeted PD-L1 on proliferation,migration,invasion and tumor stemness in hepatocellular carcinoma

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 17
Page:
3164-3171
Research Field:
Publishing date:

Info

Title:
Effect of YTHDF3 targeted PD-L1 on proliferation,migration,invasion and tumor stemness in hepatocellular carcinoma
Author(s):
SONG Wenqian1AN Yajun1ZHU Jie1LI Yuandi1SU Min12ZHAO Youbo12HU Rong13
1.Department of Histology and Embryology,School of Basic Medicine;2.National Joint Local Engineering Laboratory for Cell Engineering and Biomedicine Technique,Guizhou Province Key Laboratory of Regenerative Medicine,Key Laboratory of Adult Stem Cell Translational Research(Chinese Academy of Medical Sciences);3.Translational Medicine Research Center,Key Laboratory of Autoimmune Disease Research in Guizhou Colleges and Universities,Guizhou Medical University,Guizhou Guiyang 550025,China.
Keywords:
hepatocellular carcinomaYTHDF3m6Atumor stemnessimmune escape
PACS:
R735.7
DOI:
10.3969/j.issn.1672-4992.2024.17.002
Abstract:
Objective:To investigate the expression level of YTHDF3 in hepatocellular carcinoma and the effects of YTHDF3 on proliferation,migration,invasion and tumor stemness of hepatocellular carcinoma and possible targets involved.Methods:RT-PCR and Western blot were used to detect the expression level of YTHDF3 in clinical hepatocellular carcinoma tissues and paracancerous tissues,and the expression of YTHDF3 at the mRNA and protein levels was detected using normal hepatocytes and hepatocellular carcinoma cells.Interfering with the expression of YTHDF3 in hepatocellular carcinoma cells in both groups,hepatocellular carcinoma cell viability,migration and invasion ability were detected by CCK-8,scratch assay and Transwell assay.The sphere-forming ability of hepatocellular carcinoma cells and changes in CD133 stemness labeling determined by flow cytometry were observed after interfering with YTHDF3.Finally down-regulation of YTHDF3 detected changes in PD-L1 at mRNA and protein levels.Results:YTHDF3 expression was significantly up-regulated in hepatocellular carcinoma tissues compared with paracancerous tissues.Compared with LO2,YTHDF3 expression was elevated in hepatocellular carcinoma cells in both groups.Interference with YTHDF3 expression in hepatocellular carcinoma cells resulted in diminished proliferation,migration,invasion,and spheroid formation,and decreased CD133 stemness marker positivity.Down-regulation of YTHDF3 expression in hepatocellular carcinoma cells was followed by a decrease in PD-L1 expression.Conclusion:High expression of YTHDF3 in hepatocellular carcinoma promotes the proliferation,migration,invasion and stemness characteristics of hepatocellular carcinoma cells,and its role may be to achieve hepatocellular carcinoma cell immune escape by targeting PD-L1.

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Memo

Memo:
National Natural Science Foundation of China(No.32260165);国家自然科学基金(编号:32260165);贵州省科技计划项目(编号:黔科合基础-ZK〔2022〕一般404);贵州省普通高等学校青年科技人才成长项目(编号:黔教合KY字〔2022〕244号)
Last Update: 2024-07-31