|Table of Contents|

Effects of exosome-like nanovesicles from Selaginella doederleinii Hieron on proliferation,morphology,migration,invasion and apoptosis of hepatocellular carcinoma cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 16
Page:
2937-2944
Research Field:
Publishing date:

Info

Title:
Effects of exosome-like nanovesicles from Selaginella doederleinii Hieron on proliferation,morphology,migration,invasion and apoptosis of hepatocellular carcinoma cells
Author(s):
HU Ju1; LIANG Zicheng1; TAN Xiaoning2; ZHOU Fang2; ZENG Puhua2; 3
1.Hunan University of Chinese Medicine,Hunan Changsha 410208,China;2.Hunan Provincial Hospital of Integrated Traditional Chinese and Western Medicine,Hunan Changsha 410006,China;3.Cancer Institute of Hunan Academy of Traditional Chinese Medicine,Hunan Changsha 410006,China.
Keywords:
hepatocellular carcinoma; Selaginella doederleinii Hieron; exosome; traditional Chinese medicine
PACS:
R735.7
DOI:
10.3969/j.issn.1672-4992.2024.16.003
Abstract:
Objective:To investigate the effects of exosome-like nanovesicles from Selaginella doederleinii Hieron (SDHENs) on hepatocellular carcinoma cells.Methods:The refined ultra-high speed centrifugation method and sucrose density gradient centrifugation method were used to extract and purify SDHENs.It was identified by transmission electron microscopy (TEM) and nanoparticle tracking analysis (NTA).Green fluorescent probe (DIO) was used to label SDHENs,observing the uptake of SDHENs by cells.CCK-8 method was used to detect the effect of different concentrations of SDHENs on hepatocellular carcinoma cells,and the half inhibitory concentration (IC50) of HepG2 cells was calculated.The effect of SDHENs on the morphological changes of different hepatocellular carcinoma cells was observed.The effects of SDHENs on the proliferation,migration,invasion and apoptosis of hepatocellular carcinoma cells were observed by colony-formation assay,wound healing,Transwell invasion assay and flow cytometry.Results:It was proved that the extracts were exosome-like nanovesicles,and the SDHENs could significantly inhibit the viability of hepatocellular carcinoma cells,inhibit the proliferation,migration and invasion of hepatocellular carcinoma cells,and promote cell apoptosis(P＜0.05).Conclusion:The SDHENs have certain inhibitory effect on hepatocellular carcinoma cells,and may cooperate with cisplatin to play an inhibitory and pro-apoptotic role.

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Memo

Memo:
国家中医药管理局首批全国青年岐黄学者人才项目(编号：国中医药人教发【2020】7号)；湖南省重点研发项目（编号：2021SK2006,2023SK2057）；湖南省自然基金项目（编号：2021JJ30417,2023JJ40403）；湖南中医药大学研究生创新课题（编号：2023CX113）；湖南省中医药研究院科研课题（编号：202011）
Last Update: 1900-01-01