|Table of Contents|

The AKT/mTOR and MAPK pathway are involved in the induction of autophagy by Cucurbitacin B in non-small cell lung cancer cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 16
Page:
2929-2936
Research Field:
Publishing date:

Info

Title:
The AKT/mTOR and MAPK pathway are involved in the induction of autophagy by Cucurbitacin B in non-small cell lung cancer cells
Author(s):
CUI Qidi1; LYU Guangyao2; ZHANG Jinjie2; SUN Shanshan1; LU Mei2; 3; LYU Wenwen2; 3
1.Department of Oncology,Binzhou Medical University Hospital,Shandong Binzhou 256600,China;2.Department of Pharmacy,Binzhou Medical University Hospital,Shandong Binzhou 256600,China;3.School of Pharmacy,Binzhou Medical University,Shandong Yantai 264600,China.
Keywords:
Cucurbitacin B; NSCLC; autophagy; AKT; mTOR; MAPK
PACS:
R734.2
DOI:
10.3969/j.issn.1672-4992.2024.16.002
Abstract:
Objective:To examine the impact of Cucurbitacin B (CuB) on autophagy in non-small cell lung cancer cells and explore its underlying mechanism.Methods:CCK-8 method was used to determine the effect of CuB on the proliferation of A549 cells and H1299 cells.Autophagy was observed using fluorescent staining with monodansylcadaverine(MDC) under a fluorescence microscope，and autophagosomes were observed using transmission electron microscopy.In addition,the changes of autophagic flux after transfection of autophagy double-labeled adenovirus (mRFP-GFP-LC3) were detected by confocal microscopy.The levels of autophagy-related markers including LC3II/I,p62 and Beclin-1 were measured using Western blot.Additionally,the levels of AKT,mTOR,ERK,p38,JNK,p-AKT,p-mTOR,p-ERK,p-p38 and p-JNK were also examined.Results:CuB dose-dependently suppressed the growth of A549 and H1299 cells.In the drug treatment group，clear and dense green spots were observed after MDC staining.Transmission electron microscopy indicated the presence of autophagic lysosomes as well.After transfection of A549 and H1299 cells with mRFP-GFP-LC3,the red fluorescence was increased in CuB group.The expression levels of LC3II/I and Beclin-1 in A549 and 1299 cells were increased after CuB treatment at 0.04 μmol/L for 48 h,while that p62 was decreased.Furthermore,the expression of p-AKT and p-mTOR were decreased,but p-ERK,p-JNK and p-p38 MAPK were elevated.Conclusion:This study firstly elucidated the potential of CuB to induce autophagy in NSCLC,the mechanism might be related to inhibition of AKT/mTOR and activation of MAPK signaling pathway.

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Memo

Memo:
National Natural Science Foundation of China(No.81502937）；国家自然科学基金（编号：81502937）；吴阶平医学基金（编号：320.6750.2021-10-18）；齐鲁卫生与健康杰出青年人才项目（编号：鲁卫人才字〔2020〕3号）；山东省中医药科技发展计划项目（编号：Q-2023105）；滨州医学院科研基金（编号：BY2021KJ44）
Last Update: 1900-01-01