|Table of Contents|

Linc01419 regulates miR-34a-5p/E2F3 axis to promote proliferation and invasion of bladder cancer cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 16
Page:
2921-2929
Research Field:
Publishing date:

Info

Title:
Linc01419 regulates miR-34a-5p/E2F3 axis to promote proliferation and invasion of bladder cancer cells
Author(s):
XIANG Wei; LYU Lei; ZHENG Fuxin; YUAN Jingdong; WU Wei
Department of Urology,Wuhan No.1 Hospital,Hubei Wuhan 430022,China.
Keywords:
bladder cancer; Linc01419; miR-34a-5p; E2F3
PACS:
R737.14
DOI:
10.3969/j.issn.1672-4992.2024.16.001
Abstract:
Objective:To investigate the effect and the molecular mechanism of long non-coding RNA Linc01419 in facilitating the proliferation and invasion of bladder cancer cells.Methods:Differential expression of Linc01419 between bladder cancer tissues and normal bladder tissues was determined using the online software UALCAN (https://ualcan.path.uab.edu/).The data for this analysis was retrieved from the TCGA database.Real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) analysis was used to investigate the differential expression of Linc01419 in several types of bladder cancer cell lines,and in bladder cancer tissues and adjacent normal bladder tissues from 22 patients who received surgery and were pathologically diagnosed with bladder carcinoma.To determine the effect of Linc01419 on proliferation and invasion of bladder cancer cells,cell counting kit-8 (CCK-8） and Transwell assays were performed.Dual-luciferase reporter assay was applied to verify the targeting relation among Linc01419,miR-34a-5p and E2F3.Results:The UALCAN database analysis revealed that Linc01419 was highly expressed in bladder cancer tissues when compared to normal bladder tissues (P＜0.001).Consistent with the results of UALCAN database analysis,Linc01419 was confirmed to be up-regulated in 22 bladder cancer tissues as compared with adjacent normal bladder tissues (P＜0.001).Moreover,Linc01419 expression was higher in four types of bladder cancer cells than in normal bladder epithelial cells (P＜0.01).Silencing of Linc01419 led to a significant reduction in the proliferation and invasion of bladder cancer cells.Additionally,it resulted in a notable decrease in the expression of N-cadherin and PCNA proteins,while there was a significant increase in the expression of E-cadherin (P＜0.05).Overexpression of miR-34a-5p also exerted similar inhibitory effects on bladder cancer cells.Dual-luciferase reporter gene assay,RIP as well as Pull-down assays verified that Linc01419 can target and bind to miR-34a-5p,which further mediated the targeted regulation of E2F3.The suppression of miR-34a-5p expression substantially mitigated the influence of Linc01419 silencing on the biological characteristics of bladder cancer cells,as well as the expression levels of E-cadherin,N-cadherin,PCNA,and E2F3.Conclusion:Linc01419 is aberrantly overexpressed in bladder cancer and can promote bladder cancer cell proliferation and invasion by targeting the miR-34a-5p/E2F3 pathway,which indicates that abnormal expression of Linc01419 may act as an important procedure in carcinogenesis and development of bladder cancer.

References:

［1］ NEMETH K,BAYRAKTAR R,FERRACIN M,et al.Non-coding RNAs in disease:from mechanisms to therapeutics ［J］.Nat Rev Genet,2024,25(3):211-232.
［2］ SUN Z,TAN Z,PENG C.Long noncoding RNA LINC01419 promotes hepatocellular carcinoma malignancy by mediating miR-485-5p/LSM4 axis ［J］.Kaohsiung J Med Sci,2022,38(9):826-838.
［3］ CHENG Z,HOU S,WU Y,et al.LINC01419 promotes cell proliferation and metastasis in lung adenocarcinoma via sponging miR-519b-3p to up-regulate RCCD1［J］.Biochem Biophys Res Commun,2019,520(1):107-114.
［4］ CHEN J,LIN Z,QIN Y,et al.Overexpression of long noncoding RNA LINC01419 in esophageal squamous cell carcinoma and its relation to the sensitivity to 5-fluorouracil by mediating GSTP1 methylation ［J］.Ther Adv Med Oncol,2019,11:1758835919838958.
［5］ GU Z,WU S,WANG J,et al.Long non-coding RNA LINC01419 mediates miR-519a-3p/PDRG1 axis to promote cell progression in osteosarcoma ［J］.Cancer Cell Int,2020,20:147.
［6］ LI K,YAO T,WANG Z.lncRNA-mediated ceRNA network in bladder cancer ［J］.Noncoding RNA Res,2022,8(2):135-145.
［7］ WEI Z,LAN X,MA X,et al.Molecular mechanism of microRNAs,long noncoding RNAs,and circular RNAs regulating lymphatic metastasis of bladder cancer ［J］.Urol Oncol,2024,42(1):3-17.
［8］ GODET A,ROUSSEL E,LAUGERO N,et al.Translational control by long non-coding RNAs［J］.Biochimie,2024,217:42-53.
［9］ ALSAYED R,SHEIKHAN K,ALAM M,et al.Epigenetic programing of cancer stemness by transcription factors-non-coding RNAs interactions ［J］.Semin Cancer Biol,2023,92:74-83.
［10］ LIU S,LI J,XUE M,et al.LncRNA UCA1 participates in de novo synthesis of guanine nucleotides in bladder cancer by recruiting TWIST1 to increase IMPDH1/2［J］.Int J Biol Sci,2023,19(8):2599-2612.
［11］ LI T,HUANG M,SUN N,et al.Tumorigenesis of basal muscle invasive bladder cancer was mediated by PTEN protein degradation resulting from SNHG1 upregulation ［J］.J Exp Clin Cancer Res,2024,43(1):50.
［12］ ZHENG R,GAO F,MAO Z,et al.LncRNA BCCE4 genetically enhances the PD-L1/PD-1 interaction in smoking-related bladder cancer by modulating miR-328-3p-USP18 signaling［J］.Adv Sci (Weinh),2023,10(30):e2303473.
［13］ 孔钰琳,荣胜忠,王慧单,等.不同基因作为竞争性内源RNA参与膀胱癌调控的研究进展［J］.现代肿瘤医学,2021,29（20）:3662-3668. KONG YL,RONG SZ,WANG HD,et al.Rsearch progress of different genes as ceRNA involved in bladder cancer regulation ［J］.Modern Oncology,2021,29（20）:3662-3668.
［14］ WANG C,XIN H,YAN G,et al.NONHSAG028908.3 sponges miR-34a-5p to promote growth of colorectal cancer via targeting ALDOA［J］.Oncol Rep,2023,49(5):89.
［15］ ZHAO X,ZHUANG Y,WANG B,et al.The miR-34a-5p-c-MYC-CHK1/CHK2 axis counteracts cancer stem cell-like properties and enhances radiosensitivity in hepatocellular cancer through repression of the DNA damage response ［J］.Radiat Res,2023,199(1):48-60.
［16］ CHOU K,CHANG A,TSAI T,et al.MicroRNA-34a-5p serves as a tumor suppressor by regulating the cell motility of bladder cancer cells through matrix metalloproteinase-2 silencing［J］.Oncol Rep,2021,45(3):911-920.

Memo

Memo:
National Natural Science Foundation of China(No.81502204）；国家自然科学基金青年项目（编号:81502204）；湖北省卫健委2021-2022年度青年人才项目（编号：WJ2021Q001）
Last Update: 1900-01-01