|Table of Contents|

Basic research on thyroid cancer helps epidemiological screening

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 15
Page:
2746-2752
Research Field:
Publishing date:

Info

Title:
Basic research on thyroid cancer helps epidemiological screening
Author(s):
TANG Xiaojia; ZHAO Wenhu; Gesangzhuoga; DENG Liping
Lhasa Center for Disease Control and Prevention，Tibet Lhasa 850000，China.
Keywords:
papillary thyroid carcinoma; TCGA; target gene; epidemiological research
PACS:
R736.1
DOI:
10.3969/j.issn.1672-4992.2024.15.009
Abstract:
Objective：To screen for predictive markers of papillary thyroid carcinoma (PTC) through cancer genome mapping,and to screen for predictive indicators of papillary thyroid carcinoma through basic experimental research on marker genes,further guiding epidemiological screening.Methods：We obtained the PTC cancer tissues from The Cancer Genome Atlas (TCGA) database.Gene expression data from adjacent normal tissues were collected and bioinformatics analysis was conducted.In R software,we identified differential expression genes between thyroid cancer and normal tissues from the TCGA database with the assistance of the EdgeR software package.Then we applied STRING and Cytoscape software to carry out the gene ontology (GO) and KEGG pathway-related enrichment analysis and module analysis of differentially expressed genes.Oncomine database was used to further verify the expression differences of potential target genes selected by module analysis at the mRNA level.Finally,the protein expression levels of target genes were further validated through human protein profiling and thyroid tumor cell line protein blotting experiments,cell proliferation experiment to verify the effect of target genes on the development process of thyroid cancer cells，as well as Kaplan-Meier Plotter analysis of their impact on the prognosis of thyroid cancer.Results：A total of 747 different genes (DEGs) were screened,including 399 low-regulated DEGs and 348 up-regulated DEGs.GO analysis showed that these DEGs are rich in biological processes.KEGG pathway analysis showed that DEGs was mainly involved in hematopoietic cell lineage.The 20 major genes were identified by protein-protein interaction （PPI ）network and module analysis.Importantly,CDH2,FN1,SERPINA1 may be the target genes of PTC.The expression levels and prognosis of these genes in PTC tissues and cells were verified by the Oncomine database,human protein profiles,and Western blot experiments.We found that CDH2,FN1,and SERPINA1 were highly expressed at both mRNA and protein levels,and these target genes can all affect the proliferation of thyroid cells，and SERPINA1 predicted poor prognosis of thyroid cancer.Conclusion：SERPINA1 is significantly highly expressed in PTC tumor tissues and cells,and SERPINA1 indicates poor prognosis.This suggests that genetic testing can be used in the epidemiological screening process to identify potential patients with thyroid cancer in advance,and guide residents to conduct physical examinations to prolong patient survival.

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Last Update: 2024-06-28