|Table of Contents|

Choline kinase α mediates the malignant phenotype of glioblastoma U87MG cells through exosomes

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 15
Page:
2738-2745
Research Field:
Publishing date:

Info

Title:
Choline kinase α mediates the malignant phenotype of glioblastoma U87MG cells through exosomes
Author(s):
LI Jialin; ZOU Yourui; LIU Yang; ZHAO Yang; MA Hui
General Hospital of Ningxia Medical University，Ningxia Yinchuan 750004，China.
Keywords:
Choline kinase α; exosome; glioblastoma; proliferation; migration; invasion
PACS:
R739.41
DOI:
10.3969/j.issn.1672-4992.2024.15.008
Abstract:
Objective:To investigate the effect of Choline kinase alpha (CHKA) on the proliferation,migration and invasion of glioblastoma U87MG cells through exosomes.Methods:U87MG cells were transfected with shCHKA and its empty vector control.Exosome secretion was inhibited by exosome secretion inhibitor GW4869.Transmission electron microscopy and nanoparticle size tracing analysis were used to identify the morphology and size of exosomes.BCA method was used to identify the concentration of exosomes,and Western Blot was used to detect the expression levels of exosome marker proteins.Exosomes extracted from the cell culture supernatant of different CHKA expression groups were co-incubated with U87MG.CCK-8 assay was used to detect cell proliferation,scratch test was used to detect cell migration,and Transwell test was used to detect cell invasion.Finally,U87MG cells were used to construct a nude mouse model to observe the effect of exosomes derived from U87MG cells with different CHKA expressions on the tumorigenic ability of nude mice.Results:Compared with the empty vector control cells,the concentration of exosomes in the culture supernatant of shCHKA cells was decreased (P＜0.001),and the expression level of exosome-specific proteins was decreased (P＜0.001).After adding GW4869,the proliferation,migration and invasion ability of glioma U87MG cells were decreased (P＜0.05,P＜0.01,P＜0.01).GW4869 significantly inhibited the proliferation,migration and invasion of the empty vector control cells (P＜0.05,P＜0.001,P＜0.001),but the inhibitory effect on shCHKA cells was not obvious.Compared with U87MG cells added with exosomes from shNC group,the proliferation,migration,and invasion abilities of U87MG cells added with exosomes from shCHKA group were significantly weakened (P＜0.01,P＜0.05,P＜0.001).The tumor formation experiment in nude mice showed that the tumor growth ability of nude mice injected with exosomes from the shCHKA group was significantly reduced compared with that of the shNC group (P＜0.01).Conclusion:The expression of CHKA may mediate the malignant phenotype of glioblastoma U87MG cells through exosomes.

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Memo

Memo:
宁夏自然科学基金项目（编号：2022AAC03559）
Last Update: 2024-06-28