|Table of Contents|

Expression of Mis18β in bladder cancer tissues and its clinical significance based on multiplex bioinformatics database

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 14
Page:
2625-2629
Research Field:
Publishing date:

Info

Title:
Expression of Mis18β in bladder cancer tissues and its clinical significance based on multiplex bioinformatics database
Author(s):
CAO Wenjing1; 2; SONG Xuan2; ZHANG Zongliang3; YUAN Jiangshui2; SONG Weiqing1; 2
1.Qingdao University，Shandong Qingdao 266011,China;2.Department of Laboratory Medicine,Qingdao Municipal Hospital，Shandong Qingdao 266011,China;3.Department of Urology,Affiliated Hospital of Qingdao University,Shandong Qingdao 266011,China.
Keywords:
bladder cancer; Mis18β; TCGA database; GEO database; RT-PCR
PACS:
R737.14
DOI:
10.3969/j.issn.1672-4992.2024.14.023
Abstract:
Objective:To investigate the expression,clinical significance and role of Mis18β gene in bladder cancer.Methods:The Cancer Genome Atlas (TCGA) database obtained the RNA sequencing data and clinical data of Mis18β expression in patients with bladder cancer,and the Mis18β expression data of bladder cancer were obtained through the Gene Expression Omnibus (GEO) database,and the differences in mRNA expression of Mis18β in bladder cancer tissues and adjacent tissues were analyzed.The data were processed by bioinformatics methods,statistically analyzed their correlation with the clinicopathological parameters of patients and performed survival analysis,and the possible signaling pathways of Mis18β in the occurrence and development of bladder cancer were explored by functional analysis of Gene Ontology (GO) and enrichment analysis of the Kyoto Encyclopedia of Genes and Genomes (KEGG).Peripheral blood samples from bladder cancer patients and healthy controls were collected,and the RT-PCR method was used to detect the difference in Mis18β mRNA expression levels.Results:The analysis of TCGA database and GEO database showed that the expression of Mis18β in the bladder cancer group was significantly higher than that in the control group,and the difference was statistically significant (P＜0.05).Survival analysis showed that the overall survival of bladder cancer patients in the high-expression group of Mis18β was significantly lower than that of the low-expression group (P＜0.05).Functional enrichment analysis showed that Mis18β was mainly involved in cell mitosis,regulating cell cycle and DNA replication.The content of Mis18β in peripheral blood nucleated cells in bladder cancer patients was significantly higher than that in the control group (P＜0.05).Conclusion:Mis18β is highly expressed in bladder cancer,and its expression level is correlated with clinicopathological features such as age,pathological type,and overall survival,which provides a theoretical basis for revealing the molecular mechanism of bladder cancer occurrence and development and finding new diagnostic and prognostic markers.

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Memo

Memo:
National Natural Science Foundation of China(No.31971191）；国家自然科学基金(编号：31971191)
Last Update: 1900-01-01