|Table of Contents|

Preliminary study on the role of the lncRNA AL022344.4 in promoting invasion and migration of lung adenocarcinoma cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 14
Page:
2523-2529
Research Field:
Publishing date:

Info

Title:
Preliminary study on the role of the lncRNA AL022344.4 in promoting invasion and migration of lung adenocarcinoma cells
Author(s):
NI Linfeng1; WANG Wei1; PANG Min2; LIU Chaofeng2; WANG Hailong1; SUN Yan3
1.Institute of Cancer Biology,Basic Medical Sciences Center,School of Basic Medicine,Shanxi Medical University,Shanxi Jinzhong 030600,China;2.Shanxi Province Key Laboratory of Respiratory Disease Control,Department of Respiratory and Critical Care Medicine,the First Hospital,Shanxi Medical University,Shanxi Taiyuan 030001,China;3.Department of Management,Shanxi Medical University,Shanxi Jinzhong 030600,China.
Keywords:
lung adenocarcinoma; lncRNA AL022344.4; invasion; migration; ZEB1; EMT; MMP9
PACS:
R734.2
DOI:
10.3969/j.issn.1672-4992.2024.14.005
Abstract:
Objective:To investigate the impact of the long non-coding RNA (lncRNA) AL022344.4 on the invasion and migration of human lung adenocarcinoma cells H157 and A549.Methods:Two publicly available datasets from TCGA_LUAD and GTEx_LUNG were selected for bioinformatics analysis of lncRNA AL022344.4 expression.qRT-PCR was used to detect the relative expression of AL022344.4 in human normal bronchial epithelial cells (HBEC) and several lung adenocarcinoma cells (H157,A549,H1299,H1975,CALU-3).Lentiviral infection and plasmid transfection were employed to establish AL022344.4 knockdown in H157 cells and AL022344.4 overexpression in A549 cells.qRT-PCR was performed to confirm AL022344.4 expression in both cell lines.Cell scratch and Transwell experiments were conducted to assess the impact of AL022344.4 knockdown or overexpression on the migration and invasion of H157 and A549 cells.Western blot analysis was used to measure the protein levels of E-Cadherin,N-Cadherin,Vimentin,α-SMA,MMP9,and ZEB1.Results:Bioinformatics analysis revealed higher expression of lncRNA AL022344.4 in lung adenocarcinoma tissues compared to matched adjacent tissues.qRT-PCR confirmed its elevated expression in lung adenocarcinoma cells compared to HBEC cells,with statistical significance (P＜0.01).qRT-PCR further demonstrated that the AL022344.4 knockdown H157 cells or AL022344.4 overexpression A549 cells were successfully constructed.Cell scratch and Transwell experiments showed that AL022344.4 overexpression promoted migration and invasion of A549 cells,while AL022344.4 knockdown inhibited migration and invasion of H157 cells.Western blot indicated that AL022344.4 overexpression increased the protein levels of N-Cadherin,α-SMA,Vimentin,MMP9,and ZEB1,while reduced E-Cadherin level.Conversely,AL022344.4 knockdown enhanced E-Cadherin level,but decreased the protein levels of N-Cadherin,Vimentin,α-SMA,MMP9,and ZEB1.Conclusion:lncRNA AL022344.4 promotes the expression of ZEB1 to regulate E-Cadherin,N-Cadherin,Vimentin,α-SMA,and collaboratively with MMP9,enhances the invasion and migration of lung adenocarcinoma cells.

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Last Update: 1900-01-01