|Table of Contents|

Recombinant Newcastle disease virus rL-RVG induces lung adenocarcinoma PC9 cell pyroptosis by modulating the NLRP3/Caspase1/GSDMD pathway

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 14
Page:
2518-2523
Research Field:
Publishing date:

Info

Title:
Recombinant Newcastle disease virus rL-RVG induces lung adenocarcinoma PC9 cell pyroptosis by modulating the NLRP3/Caspase1/GSDMD pathway
Author(s):
LI Yang1; HE Yingjue1; GONG Kewen1; TIAN Yidu1; WANG Chen1; YAN Yulan1; 2
1.Department of Respiratory Medicine,People's Hospital Affiliated to Jiangsu University,Jiangsu Zhenjiang 212000,China;2.Department of Respiratory Medicine,the University of Hong Kong-Shenzhen Hospital,Guangdong Shenzhen 518000,China.
Keywords:
genetically engineered virus; lung adenocarcinoma cells; cell proliferation; cell migration; cell pyroptosis
PACS:
R734.2
DOI:
10.3969/j.issn.1672-4992.2024.14.004
Abstract:
Objective:To investigate the effect of genetically engineered virus rL-RVG on lung adenocarcinoma cells (PC9) and its underlying mechanisms.Methods:Cell proliferation ability was assessed by CCK8 assay,and cell migration and invasion ability was detected by Transwell assay.NLRP3,Caspase1,GSDMD,Asc and IL-1β protein expression was analysed by Western blotting,and NLRP3 expression was detected by immunofluorescence staining.Hoechst 33342/PI double staining was applied to identify the pyroptosis.The effect of blocking the NLRP3/Caspase1/GSDMD pathway on the pyroptosis was verified using the NLRP3 inhibitor MCC950 pretreatment.Results:After rL-RVG treatment,PC9 cells exhibited morphological changes of cellular pyroptosis,including cell swelling,bubble protrusion,membrane rupture,and cytoplasmic atrophy.rL-RVG treatment significantly inhibited the proliferation of PC9 cells,and the inhibition effect exceeded that of conventional NDV treatment.Western blotting analysis showed that NLRP3,Caspase1-p20,GSDMD-NT,Asc and IL-1β protein expressions were significantly up-regulated in the NDV,rL-RVG-treated groups,and these up-regulatory effects were more significant in the rL-RVG group.Immunofluorescence staining revealed that the expression of NLRP3 was higher in the rL-RVG group than in the NDV and Mock groups.In addition,rL-RVG treatment significantly attenuated the migration and invasion ability of PC9 cells.Hoechst 33342/PI double staining showed that rL-RVG treatment resulted in a significant increase in PI-positive cells (red fluorescence),suggesting cellular pyroptosis,with the effect being more pronounced in the rL-RVG group.The MCC950 pretreatment assay further confirmed that blocking NLRP3/Caspase1/GSDMD signalling pathway could attenuate rL-RVG-induced PC9 cell pyroptosis.Conclusion:rL-RVG inhibited lung adenocarcinoma cell proliferation and induced the pyroptosis by regulating the NLRP3/Caspase1/GSDMD pathway.

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Memo

Memo:
广东省自然科学基金（编号：2024A1515012336）；江苏省镇江市社会发展项目（编号：SH2021032）
Last Update: 1900-01-01