|Table of Contents|

Regulation of Ndfip1 expression by miR-155-5p affects the malignant biological behavior of breast cancer cells and its related mechanisms

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 14
Page:
2499-2505
Research Field:
Publishing date:

Info

Title:
Regulation of Ndfip1 expression by miR-155-5p affects the malignant biological behavior of breast cancer cells and its related mechanisms
Author(s):
XU Rui; QIAN Jun; ZHANG Mingliang; ZHANG Ligong; GUO Chenxu; YIN Faxiang; CHU Yunmian
Department of Oncology Surgery,the First Affiliated Hospital of Bengbu Medical University,Anhui Bengbu 233000,China.
Keywords:
breast cancer; miR-155-5p; Ndfip1; PI3K/AKT signaling pathway
PACS:
R737.9
DOI:
10.3969/j.issn.1672-4992.2024.14.001
Abstract:
Objective:To explore the effect of miR-155-5p on the proliferation,apoptosis,invasion and migration of breast cancer cells by targeting Ndfip1 and the related mechanisms.Methods:Real-time fluorescence quantitative polymerase chain reaction (qRT-PCR) was used to detect miR-155-5p and Ndfip1 gene expressions in 25 cases of breast cancer with definite pathological diagnosis and corresponding adjacent normal tissues.MCF-7 cells were selected.Cells of Control group,NC-inhibitor group,miR-155-5p inhibitor group,miR-155-5p inhibitor+si-NC group and miR-155-5p inhibitor+si-Ndfip1 group were constructed.CCK8 assay,flow cytometry,Transwell assay and scratch assay were used to detect changes in cell proliferation,apoptosis,invasion and migration ability,respectively.Western blot assay was used to detect protein expression related to PI3K/AKT signaling pathway.The downstream target genes of miR-155-5p were predicted by Targetscan bioinformatics website,and the correlation between miR-155-5p and target gene Ndfip1 was detected by dual luciferase assay.Results:Compared with adjacent normal tissues,the gene expression of miR-155-5p in breast cancer tissues was significantly increased (P＜0.05),and the gene expression of Ndfip1 in breast cancer tissues was significantly decreased (P＜0.05),and the expression of miR-155-5p and Ndfip1 in breast cancer tissues was negatively correlated (r=-0.969 8,P＜0.001).Compared with Control group and NC-inhibitor group,cell proliferation,invasion and migration of miR-155-5p inhibitor group were decreased (P＜0.05),apoptosis number was increased (P＜0.05),p-PI3K and p-AKT protein expression levels were decreased (P＜0.05).Dual luciferase reporter gene assay confirmed that Ndfip1 was the target of miR-155-5p.The recovery test showed that compared with the miR-155-5p inhibitor group and the miR-155-5p inhibitor+si-NC group,cell proliferation,invasion and migration of miR-155-5p inhibitor+si-Ndfip1 group were significantly increased (P＜0.05),the number of apoptosis decreased (P＜0.05),and the expression levels of p-PI3K and p-AKT protein increased (P＜0.05).Conclusion:The targeted regulation of Ndfip1 by miR-155-5p may affect the malignant biological behavior changes of breast cancer MCF-7 cells,which may be related to the expression of PI3K/AKT signaling pathway protein.

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安徽省高校自然科学研究项目（编号：2023AH051916）
Last Update: 1900-01-01