|Table of Contents|

Huaier polysaccharide inhibits proliferation and epithelial-mesenchymal transition of pancreatic cancer cells by inhibiting AKT/GSK3β/Snail signaling pathway

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 24
Page:
4536-4541
Research Field:
Publishing date:

Info

Title:
Huaier polysaccharide inhibits proliferation and epithelial-mesenchymal transition of pancreatic cancer cells by inhibiting AKT/GSK3β/Snail signaling pathway
Author(s):
LEI Lei1; GAO Yanru1; CAI Zhou1; LI Qixiong1; RAO Xiaolong2
1.Clinical Teaching and Research Office,Medical Department,Wuhan City University,Hubei Wuhan 430083,China;2.Department of Hepatobiliary and Pancreatic Surgery,China Resources Wuhan Iron and Steel General Hospital,Hubei Wuhan 430083,China.
Keywords:
Huaier polysaccharide; AKT/GSK3β/Snail; pancreatic cancer; proliferation; apoptosis; epithelial mesenchymal transformation
PACS:
R735.9
DOI:
10.3969/j.issn.1672-4992.2023.24.009
Abstract:
Objective:To investigate the effects of Huaier polysaccharide (HP) on the proliferation,apoptosis and epithelial-mesenchymal transition (EMT) of pancreatic cancer (PC) cells by regulating the serine-threonine kinase B (AKT)/glycogen synthasc kinase-3β (GSK3β)/zinc finger transcription factor (Snail) signaling pathway.Methods:Human pancreatic cancer SW1990 cells were grouped into control group (normal culture),low concentration HP group (1 μg/mL),medium concentration HP group (5 μg/mL),high concentration HP group (10 μg/mL),activator group (10 μg/mL HP+10 μmol/L AKT/GSK3β/Snail pathway activator SC79).MTT assay was applied to detect cell proliferation.The apoptosis was detected by flow cytometry.Cell adhesion test was applied to detect cell adhesion.Cell migration was detected by scratch test.Transwell chamber was applied to detect cell invasion,and Western blot was applied to detect the expression of proliferating cell nuclear antigen (PCNA),Bcl-2 associated X protein (Bax),anti apoptotic factor B cell lymphomato-2 (Bcl-2),matrix metalloproteinase-2 (MMP-2),Vimentin,N-cadherin,E-cadherin and AKT/GSK3β/Snail pathway proteins.Results:Compared with the control group,the SW1990 cell OD490 value,number of adherent cells,scratch healing rate,invasion number,the protein expression levels of PCNA,Bcl-2,MMP-2,Vimentin,N-cadherin,p-AKT/AKT,p-GSK3β/GSK3β,and Snail in the low,medium and high concentration HP groups were lower,the apoptosis rate and the protein expression levels of Bax and E-cadherin were higher (P＜0.05).Compared with high concentration HP group,the SW1990 cell OD490 value,number of adherent cells,scratch healing rate,invasion number,the protein expression levels of PCNA,Bcl-2,MMP-2,Vimentin,N-cadherin,p-AKT/AKT,p-GSK3β/GSK3β,and Snail in activator group were higher,the apoptosis rate and the protein expression levels of Bax and E-cadherin were lower (P＜0.05).Conclusion:HP may inhibit the proliferation,migration,invasion and EMT of SW1990 cells and promote apoptosis by inhibiting AKT/GSK3β/Snail signaling pathway.

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湖北省教育厅科学研究计划指导性项目(编号：B2019360)
Last Update: 1900-01-01