|Table of Contents|

EGR1 promotes drug sensitivity of colorectal cancer cells to 5-FU via the Wnt/β-catenin pathway

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 24
Page:
4531-4535
Research Field:
Publishing date:

Info

Title:
EGR1 promotes drug sensitivity of colorectal cancer cells to 5-FU via the Wnt/β-catenin pathway
Author(s):
YANG Houlai; XIA Tian
Department of Proctology,Wuhan Third Hospital,Hubei Wuhan 430060,China.
Keywords:
colorectal cancer; early growth reaction 1; Wnt/β-catenin pathway; drug resistance
PACS:
R735.3
DOI:
10.3969/j.issn.1672-4992.2023.24.008
Abstract:
Objective:To study the effect and mechanism of early growth response 1(EGR1) regulation of Wnt/β-catenin pathway on 5-FU resistance in colorectal cancer cells.Methods:Firstly,EGR1 overexpression vector was constructed and transfected into colorectal cancer cells,combined with Wnt/β-catenin pathway inhibitors.Then the cells were divided into Control group,EGR1 NC group,ov-EGR1 group,inhibitor group and ov-EGR1+inhibitor group.Cell proliferation activity was detected by CCK8.Cell apoptosis was detected by flow cytometry.mRNA expression of EGR1 was detected by qRT-PCR.Protein expression of Caspase3,EGR1 and β-catenin were detected by Western blot.The cells in each group were treated with 0,1,5,10,20,40 μmol/L 5-FU,and then the cell activity was detected by CCK8 to evaluate the change of drug sensitivity.Results:Compared with control group,EGR1 overexpression and Wnt/β-catenin pathway inhibitors inhibited cell proliferation activity (P＜0.05),increased cell apoptosis rate (P＜0.05),up-regulated EGR1 mRNA and protein expression,and up-regulated Caspase 3 protein expression (P＜0.05),down-regulated β-catenin protein expression (P＜0.05) and enhanced cell sensitivity to 5-FU (P＜0.05).Compared with inhibitor group,ov-EGR1+inhibitor group decreased cell proliferation activity (P＜0.05).increased cell apoptosis rate (P＜0.05).EGR1 mRNA expression was up-regulated (P＜0.05).EGR1 and Caspase 3 protein expression was up-regulated (P＜0.05).β-catenin protein expression was down-regulated (P＜0.05),and cell drug sensitivity was enhanced (P＜0.05).Conclusion:Upregulation of EGR1 expression can inhibit the activation of Wnt/β-catenin pathway,thereby reducing the resistance of human colon cancer cells to 5-FU and promoting the apoptosis of cancer cells.

References:

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