|Table of Contents|

Mechanism of miR-218 targeting Bmi-1 to inhibit the proliferation of lung adenocarcinoma cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 23
Page:
4301-4306
Research Field:
Publishing date:

Info

Title:
Mechanism of miR-218 targeting Bmi-1 to inhibit the proliferation of lung adenocarcinoma cells
Author(s):
JIAO Huizhen; JIN Xiaole; YUAN Meng; WANG Tianlun
Department of Emergency,the First Affiliated Hospital of Hebei North University,Hebei Zhangjiakou 075000,China.
Keywords:
lung adenocarcinoma; miR-218; Bmi-1; target genes; proliferation; transplant tumor
PACS:
R734.2
DOI:
10.3969/j.issn.1672-4992.2023.23.002
Abstract:
Objective:To investigate the inhibitory effect and mechanism of B-cell specific moloney leukemia virus insertion site 1(Bmi-1) on the proliferation of lung adenocarcinoma cells by targeting microRNA-218(miR-218).Methods:The lung adenocarcinoma cell line A549 was cultured and transfected with miR-negative control(NC) nucleotide sequence,miR-218 nucleotide sequence,NC plasmid and Bmi-1 plasmid,respectively.The cell proliferation level was detected by plate cloning test and CCK8 test,the expression of miR-218 was detected by fluorescence quantitative PCR,the expression of Bmi-1 was detected by Western blot,and the targeting relationship between miR-218 and Bmi-1 was detected by double luciferase reporter gene.Nude mice with transplanted tumor was established,lentivirus was transfected with miR-NC or miR-218 nucleotide sequence and then the volume,quality and Bmi-1 expression of transplanted tumor was detected.Results:Compared with the miR-NC group transfected with miR-NC nucleotide sequence,the miR-218 expression level and proliferation inhibition rate of A549 cells increased,and the cloning rate,Bmi-1 expression level and luciferase activity of wild type Bmi-1 plasmid decreasedin the miR-218 group transfected with miR-218 nucleotide sequence(P＜0.05).Compared with the miR-218+NC plasmid group co-transfected with miR-218 nucleotide sequence and NC plasmid,the inhibition rate of A549 cell proliferation decreased,while the clone formation rate and Bmi-1 expression level increased in the miR-218+Bmi-1 plasmid group co-transfected with miR-218 nucleotide sequence and Bmi-1 plasmid(P＜0.05).Compared with the miR-NC group transfected with miR-NC nucleotide sequence of lentivirus,the volume,quality and Bmi-1 expression level of transplanted tumor in nude mice of miR-218 group transfected with miR-218 nucleotide sequence of lentivirus decreased(P＜0.05).Conclusion:miR-218 suppresses the proliferation of lung adenocarcinoma cell by targeted downregulation of Bmi-1 expression.

References:

［1］SUNG H,FERLAY J,SIEGEL RL,et al.Global cancer statistics 2020:GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries［J］.CA Cancer J Clin,2021,71(3):209-249.
［2］LI D,SHI J,DONG X,et al.Epidemiological characteristics and risk factors of lung adenocarcinoma:A retrospective observational study from North China［J］.Frontiers in Oncology,2022,12:892571.
［3］PENG J,LIU J,LIU H,et al.Prognostic value of microRNAs in patients with small cell lung cancer:A Meta-analysis［J］.World Journal of Surgical Oncology,2022,20(1):381.
［4］XU S,WANG Q,KANG Y,et al.Long noncoding RNAs control the modulation of immune checkpoint molecules in cancer［J］.Cancer Immunol Res,2020,8(7):937-951.
［5］刘剑锋,何攀,潘德锋.miR-218靶向Bmi-1抑制急性早幼粒细胞白血病细胞增殖的研究［J］.中国实验血液学杂志,2020,28(3):815-820. LIU JF,HE P,PAN DF.MiR-218 targeting Bmi-1 inhibits proliferation of acute promyelocyticleukemia cells［J］.Chin J Exp Hematol,2020,28(3):815-820.
［6］WU Y,TIAN S,CHEN Y,et al.miR-218 inhibits gastric tumorigenesis through regulating Bmi-1/Akt signaling pathway［J］.Pathology-Research and Practice,2018,215(2):243-250.
［7］WU J,JIANG Z,XIE Y,et al.miR-218 suppresses the growth of hepatocellular carcinoma by inhibiting the expression of proto-oncogene Bmi-1［J］.Journal of BUON,2018,23(3):604-610.
［8］XIONG D,YE Y,KUANG B,et al.Prognostic value of Bmi-1 oncoprotein expression in non-small cell lung cancer［J］.Frontiers in Laboratory Medicine,2018,2(3):109-115.
［9］张苑,韩鹏凯,王静,等.miR-28靶向LASP调控TGF-β/Smad信号通路调节肺癌细胞的侵袭和转移［J］.中国免疫学杂志,2021,37(19):2338-2344. ZHANG Y,HAN PK,WANG J,et al.miR-218 targets LASP1 in regulation of TGF-β/Smad signaling pathway regulating invasion and metastasis of lung cancer cells［J］.Chin J Immun,2021,37(19):2338-2344.
［10］CHAKRABORTY S,NATH D.A study on microRNAs targeting the genes overexpressed in lung cancer and their codon usage patterns［J］.Molecular Biotechnology,2022,64(10):1095-1119.
［11］LAI X,SCHMITZ U,VERA J,et al.The role of microRNAs in cancer biology and therapy from a systems biology perspective［J］.Advances in Experimental Medicine and Biology,2022,1385:21-22.
［12］吴哲,魏志鸿,陈少华.结直肠癌组织中长链非编码RNA NEAT1和miR-218-5p的表达及其与患者预后的关系［J］.国际消化病杂志,2022,42(1):33-37,49. WU Z,WEI ZH,CHEN SH.Relationship between expression of lncRNA NEAT1 and miR-218-5p in colorectal cancer and prognosis of patients［J］.Int J Digest Dis,2022,42(1):33-37,49.
［13］ZARE A,AHADI A,LARKI P,et al.The clinical significance of miR-335,miR-124,miR-218 and miR-484 downregulation in gastric cancer［J］.Molecular Biology Reports,2018,4(6):1587-1595.
［14］ELFIKY A,MOHAMED R,ABD E,et al.Targeted delivery of miR-218 via decorated hyperbranched polyamidoamine for liver cancer regression［J］.International Journal of Pharmaceutics,2021,610:121256.
［15］赵一宁，盖俊达，李庆昌.MicroRNA-218通过EMT抑制肿瘤侵袭转移的研究进展［J］.现代肿瘤医学，2019,27（17）：3134-3137. ZHAO YN,GAI JD,LI QC.Advances in microRNA-218 inhibiting tumor invasion and metastasis through EMT［J］.Modern Oncology，2019,27（17）：3134-3137.
［16］WANG R,XUE X,WANG Y,et al.BMI1 deficiency results in female infertility by activating p16/p19 signaling and increasing oxidative stress［J］.International Journal of Biological Sciences,2019,15(4):870-881.
［17］CHEN S,LI H,CHEN S,et al.BMI1 promotes the proliferation and inhibits autophagy of breast cancer cells by activating COPZ1［J］.Clinical and Translational Oncology,2022,24(1):2166-2174.
［18］GUO Y,ZHOU G,MA Q,et al.Bmi-1 directly upregulates glucose transporter 1 in human gastric adenocarcinoma［J］.Open Life Sciences,2022,17(1):261-271.
［19］HU C,ZHANG Q,TANG Q,et al.CBX4 promotes the proliferation and metastasis via regulating BMI-1 in lung cancer［J］.Journal of Cellular and Molecular Medicine,2020,24(1):618-631.

Memo

Memo:
河北省2023年度医学科学研究课题（编号：20231456）
Last Update: 2023-10-31