|Table of Contents|

Anti-migration and invasion effect of matrix metalloproteinase inhibitor in human pancreatic cancer cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 22
Page:
4153-4157
Research Field:
Publishing date:

Info

Title:
Anti-migration and invasion effect of matrix metalloproteinase inhibitor in human pancreatic cancer cells
Author(s):
WANG Jianqiang; QIU Aiyan; ZHANG Haiwen; WU Junben; CHENG Piguang; XU Huaiyong
Binzhou People's Hospital，Shandong Binzhou 256600，China.
Keywords:
pancreatic carcinoma; migration; invasion; matrix metalloproteinase; MMI-166
PACS:
R735.9
DOI:
10.3969/j.issn.1672-4992.2023.22.010
Abstract:
Objective:To investigate the effects of MMI-166 on the migration and invasion of human pancreatic cancer cell lines PANC-1 and SW1990.Methods:PANC-1,SW1990 cells were cultured in vitro,which were treatment with different concentrations of MMI-166.Cell migration and invasion were detected by scratch assay and Transwell assay.The expressions of proteins of MMP-2 and MMP-9 were compared in different groups of PANC-1 and SW1990 cells.Results:Compared with the control group,the scratch assay and Transwell assay showed that MMI-166 decreased cell migration and invasion with increasing concentration,with statistical difference (P<0.05).Western blot and immunofluorescence showed MMI-166 significantly inhibited the expression of MMP-2 and MMP-9 protein in a dose-dependent manner,with statistical difference (P<0.05).Conclusion:MMI-166 inhibited the MMP-2 and MMP-9 activity in pancreatic cancer cells and it is useful for the regulation of pancreatic cancer cells migration and invasion.

References:

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Memo

Memo:
滨州市人民医院院级科研课题（编号：YBKTZR202106）
Last Update: 1900-01-01