|Table of Contents|

Effects of dihydroartemisinin combined with cisplatin on proliferation and apoptosis in human neuroblastoma cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 22
Page:
4129-4135
Research Field:
Publishing date:

Info

Title:
Effects of dihydroartemisinin combined with cisplatin on proliferation and apoptosis in human neuroblastoma cells
Author(s):
LIU Yanyan1; AN Qian2; HAI Bo1; BAI Qiang1; ZHANG Chuanbo1; WAN Chengliang1
1.Kunming Children's Hospital,Affiliated Children's Hospital of Kunming Medical University,Yunnan Kunming 650018,China;2.Yunnan Cancer Hospital,the Third Affiliated Hospital of Kunming Medical University,Yunnan Kunming 650118,China.
Keywords:
dihydroartemisinin; cisplatin; neuroblastoma; proliferation; apoptosis
PACS:
R739.4
DOI:
10.3969/j.issn.1672-4992.2023.22.006
Abstract:
Objective:To improve the antitumor effect of cisplatin,to test whether dihydroartemisinin (DHA) can enhance the inhibitory effect of cisplatin(CDDP) on the growth of human neuroblastoma cells when combined DHA with and cisplatin.Methods:Human neuroblastoma cell line SH-SY5Y was used as experimental cell line.The logarithmic stage tumor cells were treated with different concentrations of DHA and CDDP separately and simultaneously.Cell proliferation inhibition rate was determined by CCK-8 method,and cell cycle and apoptosis rate were determined by flow cytometry.Compusyn software was used to calculate the combination index (CI) of DHA and CDDP.The changes of apoptosis-related proteins(Bax,Bcl-2,Cleaved-PARP,PARP,Cleaved-Caspase3,Caspase3) were detected by Western Blot.Results:The IC50 value of DHA alone was 5.794 μg/mL,that of CDDP alone was 4.326 μg/mL,and that of DHA combined with CDDP was 3.253 μg/mL.DHA inhibited the proliferation of human neuroblastoma cells in a concentration-dependent manner (P<0.05) and promoted apoptosis (P<0.05).DHA combined with CDDP had a more significant effect,and the two drugs had an antagonistic effect in low dose and a synergistic effect in high dose.Conclusion:DHA enhances the antitumor effect of cisplatin on human neuroblastoma cells in vitro,and enhances the sensitivity of human neuroblastoma cells to cisplatin therapy.

References:

［1］ CHEUNG NK,ZHANG J,LU C,et al.Association of age at diagnosis and genetic mutations in patients with neuroblastoma ［J］.Jama-Journal of the American Medical Association,2012,307(10):1062-1071.
［2］ GUAN S,ZHAO Y,LU J,et al.Second-generation proteasome inhibitor carfilzomib sensitizes neuroblastoma cells to doxorubicin-induced apoptosis ［J］.Oncotarget,2016,7(46):75914-75925.
［3］ JIANG M,STANKE J,LAHTI JM.The connections between neural crest development and neuroblastoma ［J］.Current Topics in Developmental Biology,2011,94:77-127.
［4］ CHEUNG NK,DYER MA.Neuroblastoma:developmental biology,cancer genomics and immunotherapy ［J］.Nature Reviews Cancer,2013,13(6):397-411.
［5］ MALLEPALLI S,GUPTA MK,VADDE R.Neuroblastoma:An updated review on biology and treatment ［J］.Current Drug Metabolism,2019,20(13):1014-1022.
［6］ BRIZZOLARA A,GARBATI P,VELLA S,et al.Co-administration of fendiline hydrochloride enhances chemotherapeutic efficacy of cisplatin in neuro blastoma treatment ［J］.Molecules (Basel,Switzerland),2020,25(22):5234.
［7］ ABADI AJ,MIRZAEI S,MAHABADY MK,et al.Curcumin and its derivatives in cancer therapy:Potentiating antitumor activity of cisplatin and reducing side effects ［J］.Phytotherapy Research,2022,36(1):189-213.
［8］ YANAGISAWA R,KUBOTA N,HIDAKA E,et al.Cisplatin-induced nephrotoxicity in patients with advanced neuroblastoma ［J］.Pediatr Blood Cancer,2018,65(9):e27253.
［9］ KIM SJ,KIM MS,LEE JW,et al.Dihydroartemisinin enhances radiosensitivity of human glioma cells in vitro ［J］.Journal of Cancer Research and Clinical Oncology,2006,132(2):129-135.
［10］ ZHOU Q,YE F,QIU J,et al.Dihydroartemisinin induces ER stress-mediated apoptosis in human tongue squamous carcinoma by regulating ROS production ［J］.Anti-Cancer Agents in Medicinal Chemistry,2022,22(16):2902-2908.
［11］ DAMA S,NIANGALY H,DJIMDE M,et al.A randomized trial of dihydroartemisinin-piperaquine versus artemether-lumefantrine for treatment of uncomplicated Plasmodium falciparum malaria in Mali ［J］.Malaria Journal,2018,17(1):347.
［12］ DAI X,ZHANG X,CHEN W,et al.Dihydroartemisinin:A potential natural anticancer drug ［J］.International Journal of Biological Sciences,2021,17(2):603-622.
［13］ 牛高华,武华.青蒿素及其衍生物抗肿瘤作用的研究现状 ［J］.国际中医中药杂志,2009,31(02):174-175,184. NIU GH,WU H.The research status of anti-tumor effects of artemisinin and its derivatives ［J］.International Journal of Traditional Chinese Medicine,2009,31(02):174-175,184.
［14］ PACCEZ JD,DUNCAN K,SEKAR D,et al.Dihydroartemisinin inhibits prostate cancer via JARID2/miR-7/miR-34a-dependent downregulation of Axl ［J］.Oncogenesis,2019,8(3):14.
［15］ ZHANG F,MA Q,XU Z,et al.Dihydroartemisinin inhibits TCTP-dependent metastasis in gallbladder cancer ［J］.Journal of Experimental & Clinical Cancer Research,2017,36(1):68.
［16］ 陈华,孙备,潘尚哈,等.二氢青蒿素对胰腺癌生长的抑制作用 ［J］.中华外科杂志,2009,47(13):1002-1005. CHEN H,SUN B,PAN SH,et al.Study on anticancer efect of dihydroartemisinin on pancreatic cancer ［J］.Chinese Journal of Surgery,2009,47(13):1002-1005.
［17］ WANG D,ZHONG B,LI Y,et al.Dihydroartemisinin increases apoptosis of colon cancer cells through targeting Janus kinase 2/signal transducer and activator of transcription 3 signaling ［J］.Oncology Letters,2018,15(2):1949-1954.
［18］ HU YJ,ZHANG JY,LUO Q,et al.Nanostructured dihydroartemisinin plus epirubicin liposomes enhance treatment efficacy of breast cancer by inducing autophagy and apoptosis ［J］.Nanomaterials (Basel,Switzerland),2018,8(10):null.
［19］ 王双佳,孙备,潘尚哈,等.二氢青蒿素联合吉西他宾治疗胰腺癌的实验研究［J］.中华外科杂志,2010,48(07):530-534. WANG SJ,SUN B,PAN SH,et al.Experimental study of the function and mechanism combining dihydroartemisinin and gemcitabine in treating pancreatic cancer ［J］.Chinese Journal of Surgery,2010,48(07):530-534.
［20］ MARTINHO N,SANTOS TCB,FLORINDO HF,et al.Cisplatin-membrane interactions and their influence on platinum complexes activity and toxicity ［J］.Frontiers in Physiology,2018,9:1898.
［21］ 张孜睿卓,林冰洁,王齐心,等.蛋白酶体抑制剂Marizomib联合顺铂对人子宫颈癌细胞的细胞毒性及凋亡的影响［J］.临床与实验病理学杂志,2023,39(01):12-18. ZHANG ZRZ,LIN BJ,WANG QX,et al.Effect of proteasome inhibitor marizomib combined with cisplatin on cytotoxicity and apoptosis induction in human cervical cancer cells ［J］.Chinese Journal of Clinical and Experimental Pathology,2023,39(01):12-18.
［22］ FLANAGAN L,LINDNER AU,DE CHAUMONT C,et al.BCL2 protein signalling determines acute responses to neoadjuvant chemoradiotherapy in rectal cancer ［J］.Journal of Molecular Medicine,2015,93(3):315-326.
［23］ MOLDOVEANU T,FOLLIS AV,KRIWACKI RW,et al.Many players in BCL-2 family affairs ［J］.Trends in Biochemical Sciences,2014,39(3):101-111.

Memo

Memo:
云南省教育厅科学研究基金项目（编号：2022Y217）；云南省昆明市卫生科技人才培养项目［编号：2020-SW（后备）-120］；云南省昆明市卫生科研课题（编号：2022-06-02-002）
Last Update: 1900-01-01