|Table of Contents|

miR-100-5p regulates the proliferation,migration,invasion and EMT process of thyroid papillary carcinoma cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 22
Page:
4123-4129
Research Field:
Publishing date:

Info

Title:
miR-100-5p regulates the proliferation,migration,invasion and EMT process of thyroid papillary carcinoma cells
Author(s):
WEI Weiwei; GAO Shengyu; MENG Fanshi
Department of General Surgery,First Affiliated Hospital of Jiamusi University,Heilongjiang Jiamusi 154002，China.
Keywords:
miR-100-5p; papillary thyroid carcinoma; EMT
PACS:
R736.1
DOI:
10.3969/j.issn.1672-4992.2023.22.005
Abstract:
Objective:To investigate the effect of miR-100-5p on the proliferation,migration,invasion and EMT process of papillary thyroid carcinoma cells.Methods:Through the miRNA microarray datasets（GSE104006,GSE73182,GSE151180） under the GEO database,the miRNAs with the fold change ［|log2Fold Change（FC）|>1］ and adj.P.Val<0.05 were selected for intersection.Real-time fluorescent quantitative PCR（qRT-PCR） was used to detect the expression trend of miR-100-5p in PTC cell lines（TPC-1 and B-CPAP） and human normal thyroid cell line（Nthy-ori 3-1）.Synthesize miR-100-5p-related mimics（mimics-100-5p）,inhibitors（inhibitor-100-5p） and corresponding control groups（mimics-NC and inhibitor-NC）.PTC cell lines were transfected,and the effects of miR-100-5p on PTC cells were studied in depth by Edu experiment,plate cloning experiment,scratch healing experiment and Transwell experiment.The expression trends of MMP-9 and EMT-related marker proteins in the transfected mimics-100-5p,inhibitor-100-5p and corresponding NC groups were detected by Western blot experiments.Results:Three miRNA microarray databases intersected in miR-100-5p.Compared with human normal thyroid cell line Nthy-ori 3-1,miR-100-5p was lower expressed in PTC cell line.qRT-PCR verified that the transfection was successful.Edu experiment and plate cloning experiment showed that the proliferation rate of cells overexpressing miR-100-5p was significantly reduced,but the results were opposite after knocking down miR-100-5p.Scratch healing assay and Transwell assay showed that miR-100-5p overexpressed TPC-1 cells had limited migration and invasion abilities,while the results of knocking down miR-100-5p were contrary.Western blot experiments showed that after overexpressing miR-100-5p,the protein expression of MMP-9 and VIM decreased significantly,while the expression of E-cad protein increased.Knocking down miR-100-5p could reverse the result of overexpressing miR-100-5p.Conclusion:miR-100-5p negatively regulates the proliferation,migration and invasion of PTC cells.In addition,miR-100-5p is also involved in mediating the epithelial mesenchymal transition(EMT) process of PTC.

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黑龙江省卫生健康委课题（编号：2020-328）
Last Update: 1900-01-01