|Table of Contents|

Application of combined immunohistochemical detection of cytokeratin CK15,CK18 and CK19 in differential diagnosis of some skin tumors

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 21
Page:
4030-4033
Research Field:
Publishing date:

Info

Title:
Application of combined immunohistochemical detection of cytokeratin CK15,CK18 and CK19 in differential diagnosis of some skin tumors
Author(s):
ZHOU Xiaoqiu1; YAN Li2; LI Hongjing2; LI Qing2; SUN Xiyin2; WANG Dongguan2; LI Xingong2
1.Department of Pathology，Maternal and Child Health Care Hospital of Shandong Province，Shandong Jinan 250014，China；2.Department of Pathology，Dongying People's Hospital of Shandong，Shandong Dongying 257091,China.
Keywords:
basal cell carcinoma; trichoblastoma; cytokeratin; pathology; immunohistochemistry
PACS:
R739.5
DOI:
10.3969/j.issn.1672-4992.2023.21.023
Abstract:
Objective:To evaluate the expression of CK15，CK18 and CK19 in four types of skin tumors with similar histomorphology.Methods:From May 2016 to May 2019，78 cases of seborrheic keratosis，basal cell carcinoma，trichoblastoma and syringoma were selected and detected by immunohistochemistry.The expression levels of cytokeration CK15,CK18 and CK19 in the above skin tumor were observed and recorded.Results:The expression levels of three kinds of CK were different in 78 cases of skin tumors.CK15 was mainly expressed in the basal layer of epidermis，hair follicle and trichoblastoma，partially expressed in seborrheic keratosis and basal cell carcinoma，and in peripheral cells of syringoma.CK18 was expressed in sweat gland and syringoma of skin，but a little in seborrheic keratosis，basal cell carcinoma and trichoblastoma.CK19 was detected in the basal layer of epidermis，the peripheral area of skin appendages，and the above four kinds of tumors.The expression of CK15 in trichoblastoma was significantly different from that in seborrheic keratosis，basal cell carcinoma and syringoma(P<0.05).The expression of CK18 in syringoma was statistically different from that in seborrheic keratosis，basal cell carcinoma and trichoblastoma(P<0.05).The expression of CK19 in seborrheic keratosis and basal cell carcinoma was statistically different（P<0.05).Conclusion:The expression of CK15，CK18 and CK19 can be used as an important assistant method for the diagnosis and differential diagnosis of these four skin tumors with similar histomorphology.

References:

［1］许良中，杨文涛.免疫组织化学反应结果的判断标准［J］.中国癌症杂志，1996,1（4）:229-231. XU LZ，YANG WT.Criteria for determining the result of an immunohistochemical reaction［J］.Chinese Journal of Cancer,1996，1（4）：229-231.
［2］PINCELLI C，MARCONI A.Keratinocyte stem cells:Biology and clinical applications［M］.New York:Humana Press，2013:57-64.
［3］HU MS，RENNERT RC，MCARDLE A，et al．The role of stem cells during scarless skin wound healing［J］.Adv Wound Care（New Rochelle),2014,3(4):304-314.
［4］ZHANG CP，FU XB.Therapeutic potential of stem cells in skin repair and regeneration［J］.Chin J Traumatol，2008，11（4):209-221.
［5］COTSARELIS G，KAUR P，DHOUAILLY D，et al.Epithelial stem cells in:Definition，markers，localization and functions［J］.Exp Dermatol，1999，8（1):80-88.
［6］YAY A,GUKTEPE O，BEHADIR A，et al.Assessment of markers expressed in human hair follicles according to different skin regions［J］.Adv Clin Exp Med，2018,27(7):929-939.
［7］WANG X，XING Y，LI Y.Identification and characterization of hair follicle stem cells［J］.Methods Mol Biol，2018,1842:69-80.
［8］燕荣帅，裴卓，马小艮，等.常见毛囊细胞角蛋白在毛囊周期中的表达研究［J］.中华细胞与干细胞杂志（电子版）,2019,9(1):7-12. YAN RS，PEI Z，MA XG，et al.Expression patterns of common hair follicle keratins in hair cycle［J］.Chinese Journal of Cell and Stem Cell(Electronic Edition)，2019,9(1):7-12.
［9］刘青武，何秀娟，张金超，等.干细胞在皮肤创面修复应用中的研究进展［J］.中国美容医学，2019,28(6):159-164. LIU QW，HE XJ，ZHANG JC，et al.Research progress of wound repair on skin tissue with stem cells［J］.Chinese Journal of Aesthetic Medicin，2019,28(6):159-164.
［10］孙晓艳，付小兵．表皮干细胞的研究进展［J］．感染、炎症、修复，2008,9(1):52-54. SUN XY，FU XB.Advances in epidermal stem cells［J］.Infection，Inflammation，Repair,2008，9(1):52-54.
［11］周晓秋，燕丽，李红静，等.表皮干细胞标记CK15及CK19在表皮肿瘤及皮肤附属器肿瘤中的表达［Ｊ］．现代肿瘤医学，2021,29(20):3628-3631. ZHOU XQ，YAN L，LI HJ,et al.Expression of cytokeratin CK15，CK18 and CK19 in skin tumors with similar histomorphology［J］.Modern Oncology,2021，29(20):3628-3631.
［12］NANASHIM N，ITO K，ISHIKAWA T，et al.Damage of haire follicle stem cells and alteration of keratin expression in exteration radiation-induced acute alopecia［J］.Int J Mol Med，2012，30(3):579-584.
［13］BOSE A，TEH MT，MACKENZIE IC，et al．Keratin k15 as a biomarker of epidermal stem cells［J］.Int J Mol Sci，2013，14(10):19385-19398.
［14］SONG IY，BALMAIN A.Cellular reprogramming in skin cancer［J］.Seminars in Cancer Biology，2015,32:32-39.
［15］PATEL GK，YEE CF，TERUNUMA A，et al.Identification and characterization of tumor-initiating cells in human primary cutaneous squamous cell carcinoma［J］.Journal of Investigative Dermatology，2012，132(2):401-409.
［16］GAO Y，LI M，ZHANG X，et al.Isolation，culture and phenotypic characterization of human sweat gland epithelial cells［J］.Int J Mol Med，2014,34(4):997-1003.

Memo

Memo:
-
Last Update: 2023-09-28