|Table of Contents|

miR-148a-3p targets FLOT2 to promote maligration,invasion and proliferation in hepatocellular carcinoma

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 21
Page:
3921-3927
Research Field:
Publishing date:

Info

Title:
miR-148a-3p targets FLOT2 to promote maligration,invasion and proliferation in hepatocellular carcinoma
Author(s):
WANG Qianwen1; 2; LI Wenhua1; 2; LU Junxia1; 2; ZHAO Bin1; 2; GENG Yuqing1; 2; WANG Xiaofang1; 2; WU Xiangwei1; 2; 3; CHEN Xueling1; 2
1.Key Laboratory for Prevention and Treatment of Central Asian High Disease,National Health Commission,Xinjiang Shihezi 832000,China;2.Medical College of Shihezi University,Xinjiang Shihezi 832000,China;3.Department of Hepatobiliary Surgery,the First Affiliated Hospital of Shihezi University School of Medicine,Xinjiang Shihezi 832000,China.
Keywords:
hepatocellular carcinoma; miR-148a-3p; FLOT2; migration; proliferation
PACS:
R735.7
DOI:
10.3969/j.issn.1672-4992.2023.21.004
Abstract:
Objective:To investigats the effect of lipid raft signature protein 2(Flotillin2,FLOT2) on migration,invasion and proliferation of hepatocellular carcinoma and related mechanisms.Methods:The expression of FLOT2 in hepatocellular carcinoma and its effect on the biological behavior of hepatocellular carcinoma cells were confirmed by the ENCORI database and cell functional assays.miRNAs that may regulate FLOT2 were retrieved in TargetScan,and miR-148a-3p expression in hepatocellular carcinoma and its effect on the prognosis of hepatocellular carcinoma patients were analyzed in CancerMIRNome.qRT- PCR and Western Blot were performed to detect the expression level of FLOT2 in hepatocellular carcinoma after overexpression of miR-148a-3p.Dual luciferase assay was performed to verify the targeting and regulation of miR-148a-3p and FLOT2.The cells were divided into pcDNA,pcDNA-FLOT2 and pcDNA-FLOT2+miR-148a-3p mimics groups,and the migration,invasion and proliferation abilities of the three groups were measured by Transwell assay and EdU assay.Results:FLOT2 was highly expressed in hepatocellular carcinoma,and patients with high FLOT2 expression had a worse prognosis.Overexpression of FLOT2 enhanced the migration,invasion and proliferation of hepatocellular carcinoma cells.miR-148a-3p was lowly expressed in hepatocellular carcinoma tissues,correlated with the prognosis of hepatocellular carcinoma patients,and was negatively correlated with FLOT2.Dual luciferase assay verified that the targeted regulation of FLOT2 gene by miR-148a-3p.Overexpression of miR-148a-3p decreases the mRNA and protein levels of FLOT2.Overexpression of miR-148a-3p inhibited the ability of FLOT2 to promote the migration,invasion and proliferation of hepatocellular carcinoma cells.Conclusion:miR-148a-3p targeted regulation of FLOT2 gene affect the migration,invasion and proliferation of hepatoma cells.

References:

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Memo

Memo:
National Natural Science Foundation of China(No.82060297）；中国医学科学院中央级公益性科研院所基本科研业务费专项资金资助（编号：2020-PT330-003）；国家自然地区科学基金项目（编号：82060297）；兵团指导性科技计划项目（编号：2022ZD041）；兵团财政科技计划项目区域创新引导计划（编号：2021BB006）；石河子大学自主支持科研项目(编号：ZZZC202026A)
Last Update: 2023-09-28