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Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:

2023 21

Page:

3908-3913

Research Field:

Publishing date:

Info

Title:

Diallyl disulfide inhibits invasion and epithelial-mesenchymal transition in human gastric cancer cells through LIMK1-Cofilin1 pathway

Author(s):

MA Yanhua¹; 2; XIA Hong¹; LIU Fang¹; SU Jian¹; 3; XIANG Shulin¹; SU Qi¹; 3

1.Cancer Research Institute,University of South China,Hunan Hengyang 421001,China;2.Department of Oncology,Sanya Central Hospital,Hainan Sanya 572009,China;3.Hunan Clinical Research Center for Gastric Cancer Prevention and Treatment,Department of Pathology,the Second Affiliated Hospital,University of South China,Hunan Hengyang 421001,China.

Keywords:

diallyl disulfide; human gastric cancer MGC803 cells; LIMK1-Cofilin1 pathway; proliferation; migration; invasion; epithelial-mesenchymal transition

PACS:

R735.2

DOI:

10.3969/j.issn.1672-4992.2023.21.002

Abstract:

Objective:To investigate the effects of diallyl disulfide(DADS) on invasion and epithelial-mesenchymal transformation(EMT) of human gastric cancer MGC803 cells and its mechanism.Methods:In vitro and in vivo experiments were divided into MGC803 cell group and DADS treatment group.MTT,scratch test and invasion test were used to determine the effects of DADS on proliferation,migration and invasion.The expression of EMT-related proteins in LIMK1-Cofilin1 pathway was detected by Western Blot.The morphological changes of MGC803 cells were observed by phase contrast microscopy.The effects of DADS on tumor transplantation were tested in nude mice.Results:MTT showed that DADS could inhibit MGC803 cell proliferation in a time-dependent manner(P<0.05).Scratch test showed that the cell migration distance of DADS group was (59.9±1.9)μm shorter than that of MGC803 group (127.1±2.0)μm(P<0.01).Invasion test showed that DADS group had (30.0±2.6) transmembrane cells significantly decreased compared with MGC803 group (48.3±2.5)(P<0.01).Western Blot showed that after DADS treatment for 6 h,12 h,24 h and 48 h,LIMK1,p-LIMK1 and p-Cofilin1(P<0.001),Vimentin(P<0.001) and MMP-9(P＜0.001) were down-regulated in a time-dependent manner,while E-cadherin (P=0.001) and TIMP-3 were significantly up-regulated (P<0.001) in MGC803 cells respectively.Contrast microscopy showed that after DADS treatment,the fibroblast-like cells decreased and the atypia decreased significantly.In nude mice,the tumor growth of DADS group was slower than MGC803 cell group(P<0.05),the tumor weight of DADS group was lower than MGC803 cell group,the tumor inhibition rate was 60.19%(P<0.001),and the positive expression of Ki-67,Vimentin,Snail and CD34 decreased,and the expression of E-cadherin was increased.Conclusion:DADS can inhibit MGC803 cell invasion and EMT through LIMK1-Cofilin1 pathway in vitro and in vivo.

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Memo

Memo:

National Natural Science Foundation of China(No.81973532，81374013，81102854）；国家自然科学基金(编号：81973532，81374013，81102854)

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Last Update: 2023-09-28