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Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:

2023 20

Page:

3796-3800

Research Field:

Publishing date:

Info

Title:

Expression and clinical significance of LncRNA HULC in colorectal cancer（50 cases）

Author(s):

CHEN Cunbao¹; 2; XIAO Ruixue²; 3; LI Jianqi¹; 2; ZHEN Yanan¹; 2

1Department of Gastrointestinal Surgery，the Third Affiliated Hospital of Shandong First Medical University(Affiliated Hospital of Shandong Academy of Medical Sciences),Shandong Jinan 250031,China;2Gastroenterology Research Institute and Clinical Center,Shandong First Medical University,Shandong Jinan 250031,China;3Department of Pathology,the Third Affiliated Hospital of Shandong First Medical University,Shandong Jinan 250031,China.

Keywords:

LncRNA HULC; colorectal cancer; cancer biomarkers; qRT-PCR

PACS:

R735.3

DOI:

10.3969/j.issn.1672-4992.2023.20.014

Abstract:

Objective：To investigate the expression of long non-coding RNA (LncRNA) HULC in CRC tissues and paracancerous tissues,and its correlation with the clinical characteristics of CRC patients,and to evaluate its possibility as a diagnostic marker of CRC.Methods：50 patients with primary CRC who underwent radical CRC surgery and were pathologically confirmed in our hospital from January 2018 to January 2020 were collected for their cancer and adjacent tissues (≥5 cm from cancer circumference).The expression of hsa_circ_0079656 in matched CRC and adjacent tissues was analyzed using quantitative real-time fluorescent polymerase chain reaction (qRT-PCR).Paired t test was used to address the difference between CRC and adjacent tissues.One-way ANOVA was used to analyze the relationship between hsa_circ_0079656 expression and the clinicopathologic features of patients,and the ROC curve was established to evaluate the application value of hsa_circ_0079656 in the diagnosis of CRC.Results：The ΔCT value (6.59±2.26) of HULC in CRC tissue is lower than that of ΔCT value (9.04±2.60) in paracancer tissue,indicating that the true expression of HULC in cancer tissue is higher than that in paracancer tissue,the difference is statistically significant (P＜0.001).The expression of HULC in cancer tissues was correlated with the differentiation degree of CRC (P=0.014),lymph node metastasis (P=0.007) and TNM stage (P=0.027),and the difference was statistically significant.The area under ROC curve of HULC expression in CRC and paracancer tissues was 0.762,P＜0.001,the difference was statistically significant.Conclusion：HULC is overexpressed in colorectal cancer tissues,and the expression of HULC in cancer tissues is related to the differentiation degree of CRC,TNM stage and lymph node metastasis,which may have certain reference value for the diagnosis of CRC.

References:

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