|Table of Contents|

The expression of lncRNA GATA2-AS1 in renal cell carcinoma tissues and its effect on malignant biological behaviors of renal cancer cells ACHN

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 19
Page:
3577-3582
Research Field:
Publishing date:

Info

Title:
The expression of lncRNA GATA2-AS1 in renal cell carcinoma tissues and its effect on malignant biological behaviors of renal cancer cells ACHN
Author(s):
WEI Shufei; PAN Zhenhui; QI Pan; ZHANG Xiaoyu; DONG Zhiming; ZHANG Aili
Department of Urology，Fourth Hospital of Hebei Medical University，Hebei Shijiazhuang 050011,China.
Keywords:
renal cell carcinoma; long chain non-coding RNA; proliferation; migration; invasion; epithelial-mesenchymal transition(EMT)
PACS:
R737.11
DOI:
10.3969/j.issn.1672-4992.2023.19.008
Abstract:
Objective:To detect the expression of long non-coding RNAs GATA2-AS1 in renal cell carcinoma(RCC) tissues and cell lines,and discuss the relationship between its expression and clinicopathological parameters of patients,as well as to study the effect of overexpression of GATA2-AS1 on the biological behavior and epithelial-mesenchymal transition(EMT) of renal cell carcinoma ACHN.Methods:Real-time quantitative polymerase chain reaction(RT-qPCR) was applied to detect the expression of GATA2-AS1 gene in 71 cases of renal cell carcinoma tissues and their corresponding adjacent normal tissues and renal cell carcinoma cell lines(786-O,ACHN).The overexpression vector pcDNA3.1-GATA2-AS1 was established and transfected into ACHN cells,and the transfection efficiency was detected by RT-qPCR.Cell proliferation assay(MTS) and cloning formation experiment were applied to detect the effect of GATA2-AS1 gene overexpression on the proliferation ability of ACHN cells in vitro.The effects of GATA2-AS1 overexpression on the migration and invasion ability of ACHN cells in vitro were detected by scratch experiment and Transwell chamber invasion experiment.The RT-qPCR method was applied to detect the effect of GATA2-AS1 overexpression levels of relevant markers(E-cadherin,N-cadherin and vimentin) in the EMT process.Results:The relative expression of GATA2-AS1 was significantly down-regulated in both renal cancer cell lines(P<0.01),and the expression level of GATA2-AS1 in ACHN cells was the lowest.The relative expression level of GATA2-AS1 in RCC tissue was significantly lower than that in adjacent tissues(P<0.01).The expression level of GATA2-AS1 in RCC tissue was correlated with TNM stage and lymph node metastasis(P<0.05 or P<0.01).The overexpression vector pcDNA3.1-GATA2-AS1 was successfully established and transfected into renal cell carcinoma cell line ACHN.The relative expression of GATA2-AS1 in the overexpression group was significantly higher than that in the control group(P<0.01).After GATA2-AS1 was overexpressed,it can significantly inhibit the proliferation,migration and invasion ability of ACHN cells in vitro.Meanwhile,the expression level of E-cadherin increased and the expression level of mesenchymal markers N-cadherin and Vimentin decreased.Conclusion:The low expression of GATA2-AS1 in RCC tissues and renal cancer cell lines was related to TNM staging and lymphatic metastasis in patients.Overexpression of GATA2-AS1 can significantly inhibit the proliferation,migration,invasion ability and EMT process of renal cell carcinoma cells in vitro.

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Last Update: 2023-08-31