|Table of Contents|

Mechanism of miR-506-3p inhibiting malignant phenotypes of uveal melanoma by targeting SART3

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 19
Page:
3557-3565
Research Field:
Publishing date:

Info

Title:
Mechanism of miR-506-3p inhibiting malignant phenotypes of uveal melanoma by targeting SART3
Author(s):
CHEN Yu1; ZHANG Yongzhen1; WANG Yongqiang1; WANG Zongren1; HU Yingying2
1.Department of Laboratory Medicine,Xinxiang Central Hospital,Henan Xinxiang 453000,China;2.Department of Emergency,the First Affiliated Hospital of Henan University of Science and Technology,Henan Luoyang 471000,China.
Keywords:
miR-506-3p; uveal melanoma; proliferation; squamous cell carcinoma antigen 3
PACS:
R739.5
DOI:
10.3969/j.issn.1672-4992.2023.19.005
Abstract:
Objective:To explore the mechanism of miR-506-3p inhibiting uveal melanoma(UM) by targeting squamous cell carcinoma antigen 3(SART3).Methods:In this experiment,OCM-1A and MUM-213 cells were applied to construct miR-506-3p overexpression models(miR-NC group,miR-506-3p group) and SART3 knocking-down models(shNC group,shSART3 1# group,shSART3 2# group).The relationship between miR-506-3p,SART3 and poor phenotypes of UM was analyzed by bioinformatics,and the targeted relationship between the two indexes was predicted.The expressions of miR-506-3p and SART3 in APRE-19,OCM-1A and MUM-213 cells were detected by RT-PCR.CCK-8 test,scratch healing test and invasion test were used to detect the ability of cell proliferation,lateral migration and invasion.The weight and volume of tumors were detected by tumor formation assay in nude mice.The expression of SART3 protein was detected by Western Blot.The targeted relationship between miR-506-3p and SART3 was verified by dual luciferin assay.Results:The expression of miR-506-3p was down-regulated in UM cells,and overexpression of miR-506-3p could inhibit cells proliferation,migration and invasion.Up-regulating the expression of miR-506-3p could inhibit the growth of transplanted tumors in nude mice.It was predicted by database that SART3 was the target gene of miR-506-3p,which was verified by dual luciferin assay.The expression of SART3 was up-regulated in UM.Down-regulating the expression of SART3 could inhibit the proliferation,migration and invasion of UM cells.The miR-506-3p could inhibit the proliferation,migration and invasion of UM cells by targeting SART3.Conclusion:The miR-506-3p can inhibit the proliferation,migration and invasion of UM cells,and its mechanism of action may be related to down-regulating SART3 expression.

References:

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Memo:
河南省医学科技攻关计划项目（编号：2018020283）
Last Update: 2023-08-31