|Table of Contents|

Effects of SHC SH2 domain-binding protein 1 on the malignant biological behaviors of hepatocellular carcinoma cells and its mechanism

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 19
Page:
3548-3557
Research Field:
Publishing date:

Info

Title:
Effects of SHC SH2 domain-binding protein 1 on the malignant biological behaviors of hepatocellular carcinoma cells and its mechanism
Author(s):
SONG Lei1; GAO Ming2; LI He2; SUN Yuansong2; WANG Qi2
1.Emergency Department，the First Affiliated Hospital of Anhui Medical University，Anhui Hefei 230022,China;2.Department of Emergency Surgery，the Second Hospital of Anhui Medical University,Anhui Hefei 230022,China.
Keywords:
hepatocellular carcinoma; SHCBP1; KIF18A; cell stemness
PACS:
R735.7
DOI:
10.3969/j.issn.1672-4992.2023.19.004
Abstract:
Objective:To figure out the regulatory role of SHC SH2 domain-binding protein 1(SHCBP1) in the proliferation,apoptosis,invasion,migration and stemness of hepatocellular carcinoma(HCC) cells via interacting with Kinesin family member-18A(KIF18A).Methods:ENCORI data base analyzed SHCBP1 and KIF18A expression in HCC tissues,the correlation of SHCBP1 and KIF18A expression with the overall survival rate of HCC patients and the correlation between SHCBP1 and KIF18A in HCC tissues.The relationship between SHCBP1,KIF18A and clinicopathological characteristics of HCC patients was analyzed.STRING database predicted the potential binding relationship between SHCBP1 and KIF18A.Human normal hepatocyte line HHL-5 and HCC cells(Huh-7,SNU-449 and Hep3B) were cultured in vitro.RT-qPCR and Western blot examined SHCBP1 and KIF18A expression.Cell viability was estimated by CCK-8.Cell apoptosis,migration as well as invasion were respectively measured by TUNEL,wound healing and transwell assays.In vitro sphere formation assay evaluated the sphere formation ability of cells.Western blot tested the expression of apoptosis-,metastasis-associated proteins and stemness markers.Results:There were significant correlations between SHCBP1 and KIF18A expression and the degree of tumor differentiation,vascular invasion and distant metastasis in HCC patients(all P<0.05).SHCBP1 and KIF18A were highly expressed.SHCBP1 knockdown hindered the proliferation,migration,invasion and stemness while potentiated the apoptosis of HCC cells(all P<0.01).Additionally,SHCBP1 interacted with KIF18A.Further,KIF18A over expression reversed the impacts of SHCBP1 interference on HCC cell proliferation,apoptosis,migration,invasion and stemness(all P<0.01).Conclusion:SHCBP1 might interact with KIF18A to further contribute to the malignant biological behaviors of HCC cells.

References:

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安徽省教育厅科学研究项目（编号：KJ2021A0322）
Last Update: 2023-08-31