|Table of Contents|

Midazolam inhibits the malignant biological behavior of ovarian cancer SKOV3 cells by down-regulating the expression of miR-4295

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 19
Page:
3534-3539
Research Field:
Publishing date:

Info

Title:
Midazolam inhibits the malignant biological behavior of ovarian cancer SKOV3 cells by down-regulating the expression of miR-4295
Author(s):
YI Xuecai; QI Ling; LIU Wei; QI Haiyan; MENG Fanqing
Department of Anesthesiology,Jinan Maternal and Child Care Hospital,Shandong Jinan 250001,China.
Keywords:
ovarian cancer; midazolam; miR-4295; cell proliferation; migration; invasion
PACS:
R737.31
DOI:
10.3969/j.issn.1672-4992.2023.19.002
Abstract:
Objective:To explore the effect and mechanism of midazolam on the malignant biological behavior of ovarian cancer SKOV3 cells.Methods:The human ovarian cancer cell SKOV3 in vitro were cultured and divided into Con group,L-MDZ group,M-MDZ group,H-MDZ group,anti-miR-NC group,anti-miR-4295 group,H-MDA+miR-NC group,H-MDA+miR-4295 group.CCK-8 method and plate clone formation test were used to detect cell proliferation.Scratch test and Transwell test were used to detect cell migration and invasion,respectively.qRT-PCR method was used to detect the expression of miR-4295 in ovarian cancer tissues,normal ovarian tissue and SKOV3 cells.Western blot method was used to detect the expressions of E-cadherin and N-cadherin protein.Results:Compared with the Con group,the cell proliferation inhibition rate and the protein level of E-cadherin in the L-MDZ,M-MDZ,and H-MDZ groups were increased(P<0.05),while the number of cell clone formation and the number of invaded cells were decreased(P<0.05),the expression of miR-4295,scratch healing rate and the protein level of N-cadherin were decreased(P<0.05),and were dose-dependent.Compared with normal ovarian tissues,the expression of miR-4295 in ovarian cancer tissues was increased(P<0.05).Compared with the anti-miR-NC group,the cell proliferation inhibition rate and E-cadherin protein level in the anti-miR-4295 group were increased(P<0.05),while the number of cell clone formation and the number of invasive cells were decreased(P<0.05),the rate of scartch healing and N-cadherin protein level were decreased(P<0.05).Compared with the H-MDA+miR-NC group,the cell proliferation inhibition rate and E-cadherin protein level in the H-MDA+miR-4295 group were decreased(P<0.05),while the number of cell clones and the number of invasive cells were increased(P<0.05),the scratch healing rate and N-cadherin protein level were increased(P<0.05).Conclusion:Midazolam could reduce the proliferation,migration and invasion of ovarian cancer cells by down-regulating the expression of miR-4295.

References:

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Last Update: 2023-08-31