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Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:

2023 19

Page:

3529-3534

Research Field:

Publishing date:

Info

Title:

Effect of circCSNKIG1 targeting miR-1180-5p on the biological characteristics of breast cancer cells

Author(s):

SUN Xiaohu¹; 2; 3; 4; LIU Yan⁵; CAO Xuchen¹; 2; 3; 4

Keywords:

breast cancer; circCSNKIG1; miR-1180-5p; proliferation; migration; invasion

PACS:

R737.9

DOI:

10.3969/j.issn.1672-4992.2023.19.001

Abstract:

Objective:To explore the effect of circCSNKIG1 targeting miR-1180-5p on the biological characteristics of breast cancer cells.Methods:Expression of circCSNKIG1 and miR-1180-5p in breast cancer tissues and adjacent tissues was detected by RT-qPCR.si-circCSNKIG1,pcDNA-circCSNKIG1,miR-1180-5p mimic,si-circCSNKIG1+anti-miR-1180-5p and their corresponding negative controls(si-NC,pcDNA,miR-NC and si-circCSNKIG1+anti-miR-NC) were transfected into MDA-MB-231 cells respectively.And then the wound healing,CCK-8,Transwell,and colony formation assays were applied to detect the healing rate,viability,invasions number and clone number.Luciferase reporter gene method was used to determine the targeting relationship between circCSNKIG1 and miR-1180-5p.Results:Compared with adjacent normal tissues,circCSNKIG1 expression was higher and miR-1180-5p expression was lower in breast cancer tissues(P<0.05).Interference with the expression of circCSNKIG1 significantly reduced the cell optical density(OD) value,invasion numbers,clone numbers and scratch healing rate(P<0.05).Overexpression of miR-1180-5p significantly reduced the cell OD value,invasion numbers,clone numbers and scratch healing rate(P<0.05).circCSNKIG1 targeted miR-1180-5p,and circCSNKIG1 could negatively regulate miR-1180-5p expression.Downregulating of miR-1180-5p significantly reversed the effect of interfering with circCSNKIG1 on the OD value,invasion numbers,clone numbers and scratch healing rate(P<0.05).Conclusion:Interference with circCSNKIG1 inhibits breast cancer cell proliferation,migration and invasion via increasing miR-1180-5p expression.

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Memo

Memo:

National Natural Science Foundation of China（No.82274221）;国家自然科学基金（编号：82274221）；天津市医学重点学科（专科）建设项目（编号：TJYXZDXK-009A）

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