|Table of Contents|

Expression of TRMT1 in prostate cancer tissues and its correlation with immune cell infiltration

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 18
Page:
3455-3460
Research Field:
Publishing date:

Info

Title:
Expression of TRMT1 in prostate cancer tissues and its correlation with immune cell infiltration
Author(s):
YUAN Chao1; WANG Lei2; WENG Xiaodong2; LI Mengbo1; LIU Xiuheng2; LI Youkong1
1.Department of Urology,Jingzhou Central Hospital，Hubei Jingzhou 434020，China;2.Department of Urology，Renmin Hospital of Wuhan University，Hubei Wuhan 430060，China.
Keywords:
tRNA methyltransferase 1; prostate cancer; immune cell infiltration; bioinformatics
PACS:
R737.25
DOI:
10.3969/j.issn.1672-4992.2023.18.023
Abstract:
Objective：To analyze the expression of tRNA methyltransferase 1 (TRMT1) in prostate cancer(PCa) tissues and its correlation with immune cell infiltration based on multiple bioinformatic databases and immunohistochemistry.Methods:The differential expressions of TRMT1 mRNA in PCa tissues and normal prostate tissues were analyzed by using UALCAN database.The paraffin－embedded specimens of 52 patients with PCa and 20 patients with benign prostatic hyperplasia (BPH) who underwent surgery in the department of urology of our hospital from January 2019 to June 2021 were collected.The differential expression of TRMT1 protein in PCa and BPH tissues was analyzed by immunohistochemistry.The correlation between the expression of TRMT1 protein and the clinicopathological characteristics of PCa patients was analyzed according to the results of immunohistochemistry and clinical medical records.The LinkedOmics database was used to search for co-expressed genes associated with TRMT1,and the Metascape database was used for enrichment analysis.The TRMT1 protein-protein interaction network was constructed by String database.The correlation between TRMT1 expression and immune cell infiltration was analyzed by TIMER2.0 database.The correlation between TRMT1 expression and prognosis of PCa patients was analyzed by GEPIA database.Results:Compared with normal prostate tissues,the expression of TRMT1 mRNA was higher in PCa tissues (P＜0.05).Immunohistochemical results showed that the expression of TRMT1 protein in PCa tissues was significantly higher than that in BPH tissues (P＜0.05).The expression of TRMT1 was correlated with the Gleason score of PCa patients (P＜0.05),but not with age,preoperative PSA level and TNM stage (P＞0.05).The results of enrichment analysis showed that TRMT1-related genes were mainly related to RNA metabolism and RNA modification.The expression of TRMT1 was negatively correlated with the level of immune cell infiltration of CD8+ T cells,CD4+ T cells,B cells,neutrophils,macrophages and myeloid dendritic cells.In PCa patients,the overall survival (OS) and disease free survival (DFS) of TRMT1 high expression group were significantly lower than those of TRMT1 low expression group (P＜0.05).Conclusion:TRMT1 is highly expressed in PCa and is involved in the regulation of immune cell infiltration,which is associated with poor prognosis of PCa patients and may become a new biomarker for PCa diagnosis,treatment and prognosis.

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Memo

Memo:
National Natural Science Foundation of China(No.81972408）；国家自然科学基金面上项目（编号：81972408）
Last Update: 1900-01-01