|Table of Contents|

The expression of lncRNA LINC00460 in colorectal cancer tissues and its impact on cell proliferation,invasion and migration ability

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 18
Page:
3405-3411
Research Field:
Publishing date:

Info

Title:
The expression of lncRNA LINC00460 in colorectal cancer tissues and its impact on cell proliferation,invasion and migration ability
Author(s):
LI Qing1; CUI Facai1; YANG Hongjie2; ZHAO Weifeng2
1.Department of Clinical Laboratory;2.Cancer Center,Henan Provincial People's Hospital,People's Hospital of Zhengzhou University,Henan Zhengzhou 450003,China.
Keywords:
long non-coding RNA LINC00460; colorectal cancer; proliferation; invasion; migration
PACS:
R735.3
DOI:
10.3969/j.issn.1672-4992.2023.18.013
Abstract:
Objective:To investigate the expression of long non-coding RNA (lncRNA) LINC00460 in colorectal cancer (CRC) tissues,and its effects on the proliferation,invasion and migration of CRC cells.Methods:The expression of lncRNA LINC00460 in CRC tissues,CRC cell lines(HCT-8,HCT-116,SW480,SW620) and colorectal mucosal epithelial cells NCM460 was analyzed by using the Gene Expression Profiling Interactive Analysis (GEPIA) database and quantitative polymerase chain reaction (qPCR).The correlation between the expression level of LINC00460 and clinicopathological parameters of CRC patients was further analyzed.After HCT-8 and SW620 cells were transfected with pcDNA3.1-LINC00460,pcDNA3.1-NC,si-LINC00460 or si-NC,the proliferation,invasion and migration of CRC cell lines (HCT-8 and SW620) was detected by CCK-8 assay,clonal formation assay,and Transwell assay,respectively.And the Western blot assay was used to detect the expressions of cyclin D1,p21,E-cadherin,N-cadherin and Vimentin.Results:Compared with para-carcinoma tissues and NCM460 cells,lncRNA LINC00460 was highly expressed in CRC tissues and CRC cell lines (HCT-8,HCT-116,SW480,SW620) (P＜0.05),and the high level of LINC00460 was significantly correlated with tumor differentiation,TNM stage,lymph node metastasis and Ki-67 expression (all P＜0.05).Down-regulated the expression of lncRNA LINC00460 can increase the expression of p21 and E-cadherin,decrease the expression of cyclin D1,N-cadherin and Vimentin,and inhibit the proliferation,invasion and metastasis of SW620 cells.Up-regulated expression of lncRNA LINC00460 can decrease the expression of p21 and E-cadherin,increase the expression of cyclin D1,N-cadherin and Vimentin,and promoted the proliferation,invasion and metastasis of HCT-8 cells.Conclusion:lncRNA LINC00460 is highly expressed in CRC tissues and cells,and its expression is significantly correlated to the clinical parameters of CRC patients.Up-regulated or down-regulated the expression of LINC00460 could correspondingly promote or inhibit the proliferation,invasion and migration of CRC cells.

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Memo

Memo:
National Natural Science Foundation of China(No.81802094）；国家自然科学基金课题项目（编号：81802094）
Last Update: 1900-01-01