|Table of Contents|

Ginsenoside Rd affects the proliferation and apoptosis of colon cancer SW480 cells by regulating the expression of lncRNA MAGI1-IT1

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 18
Page:
3371-3375
Research Field:
Publishing date:

Info

Title:
Ginsenoside Rd affects the proliferation and apoptosis of colon cancer SW480 cells by regulating the expression of lncRNA MAGI1-IT1
Author(s):
KOU Weizheng; WANG Fang; ZHAO Hongke; ZHANG Lin; HUANG Haibo; KOU Xiaoge
Department of Oncology，the First Affiliated Hospital of Xinxiang Medical College,Henan Weihui 453100,China.
Keywords:
ginsenoside Rd; lncRNA MAGI1-IT1; colon cancer; proliferation; apoptosis
PACS:
R735.3
DOI:
10.3969/j.issn.1672-4992.2023.18.007
Abstract:
Objective:To investigate the effect of ginsenoside Rd on the proliferation and apoptosis of colon cancer SW480 cells and its molecular mechanism.Methods:Real-time fluorescent quantitative PCR (RT-qPCR) was used to detect the expression of lncRNA MAGI1-IT1 in colon cancer tissues and cells.Colon cancer cells SW480 were divided into control group,low,medium and high dose ginsenoside Rd group,si-MAGI1-IT1 group,si-NC group,high-dose ginsenoside Rd+pcDNA-MAGI1-IT1 group,high-dose ginsenoside Rd+pcDNA group.Tetramethylazolium salt colorimetric method (MTT),flow cytometry to detect cell proliferation activity and apoptosis.Western blot method to detect proliferation (CyclinD1,p21) and apoptosis-related protein (Caspase3,Bcl-2,cleaved Caspase3,Bax) expression.Results:The expression of MAGI1-IT1 in colon cancer tissue was increased (P＜0.05).After medium and high doses of ginsenoside Rd were treated with SW480 cells,the ratio of G0/G1 phase,apoptosis rate were increased,ratio of S phase was decreased,decreased the protein expression levels of CyclinD1,Caspase3 and Bcl-2,and the expression levels of p21,increased the expression levels of cleaved-Caspase3 and Bax,and the expression of MAGI1-IT1 was decreased,in a dose-dependent manner (P＜0.05).After down-regulating lncRNA MAGI1-IT1,the ratio of G0/G1 phase and apoptosis rate were increased,ratio of S phase was decreased,decreased the protein expression levels of CyclinD1,Caspase3 and Bcl-2,and increased the expression levels of p21,cleaved-Caspase3 and Bax (P＜0.05).Up-regulation of lncRNA MAGI1-IT1 reversed the effect of ginsenoside Rd on the proliferation and apoptosis of colon cancer SW480 cells.Conclusion:Ginsenoside Rd inhibits the proliferation of colon cancer SW480 cells and promotes their apoptosis by down-regulating the expression of lncRNA MAGI1-IT1.

References:

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