|Table of Contents|

Mechanical studies of the effects of circ-BAX.3 and circ-BAX.11 in inhibiting acute myeloid leukemia cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 18
Page:
3359-3365
Research Field:
Publishing date:

Info

Title:
Mechanical studies of the effects of circ-BAX.3 and circ-BAX.11 in inhibiting acute myeloid leukemia cells
Author(s):
XING Zengwen1; WANG Wenfang1; SHI Danni2; LI Jiawei1; YAO Qi1; HAN Yanmei1; ZHANG Zhengyi3
1.Haikou of the Maternal and Child Health Hospital,Hainan Haikou 570203,China;2.Department of Clinical Laboratory Examination；3.Department of Internal Medicine-Oncology,the 928th Hospital of the Joint Logistics Support Force of the Chinese People's Liberation Army,Hainan Haikou 570203,China.
Keywords:
acute myeloid leukemia; circRNA-BAX.3; circRNA-BAX.11; miR-128; miR-214; BAX
PACS:
R733.71
DOI:
10.3969/j.issn.1672-4992.2023.18.005
Abstract:
Objective:To explore the regulatory relationship between circRNA-BAX.3 (hsa_circ_0051799),circRNA-BAX.11 (hsa_circ_0051800),miR-128,miR-214 and BAX in human acute myeloid leukemia (AML) cells proliferation and apoptosis.Methods:Real-time fluorescent quantitative PCR (qRT-PCR) was used to detect the expression levels of circRNA-BAX.3,circRNA-BAX.11,miR-128,miR-214 and BAX in peripheral blood samples and cell lines.Western-blot (WB) was used to detect the expression level of BAX protein.CCK-8 and flow cytometry were used to detect cell proliferation and apoptosis.The target relationship between BAX 3' UTR and miR-128 and miR-214 was analyzed by dual luciferase report.Results:circRNA-BAX.3 and circRNA-BAX.11 were down-regulated in AML samples and cells,and both can promote the proliferation of AML cells and inhibit cell apoptosis,and the difference was statistically significant (P＜0.05).This study found a co-regulatory network of circRNA-BAX.3,circRNA-BAX.11,miR-128,miR-214,and BAX.Further bioinformatics found that BAX was the target gene of miR-128 and miR-124.miR-128 and miR-214 can significantly down-regulate BAX expression,promote cell proliferation and inhibit cell apoptosis (P＜0.05),while circ-BAX.3 and circ-BAX.11 promote BAX by binding to miR-128 and miR-214 expresses and accelerates apoptosis in AML cells.Conclusion:circRNA-BAX.3 and circRNA-BAX.11 inhibit the progression of AML by inhibiting miR-128 and miR-214 and promoting the expression of BAX.

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