|Table of Contents|

miR-24-3p expressed in osteosarcoma and inhibited the proliferation,invasion and migration of osteosarcoma cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 18
Page:
3353-3359
Research Field:
Publishing date:

Info

Title:
miR-24-3p expressed in osteosarcoma and inhibited the proliferation,invasion and migration of osteosarcoma cells
Author(s):
WAN Ningjun1; TAO Yamei2; HAO Bin1; XU Shaowei1; WANG Zhen1
1.Department of Spinal and Bone Tumour;2.Department of Coronary Care Unit,People's Hospital of Ningxia Hui Autonomous Region，Ningxia Yinchuan 750002,China.
Keywords:
osteosarcoma; miR-24-3p; cell proliferation; cell migration and invasion
PACS:
R738.1
DOI:
10.3969/j.issn.1672-4992.2023.18.004
Abstract:
Objective:To investigate the expression of miR-24-3p in osteosarcoma and the role of regulating the biological behavior of osteosarcoma cells.Methods:Ten osteosarcoma specimens and paired para-cancer tissue specimens from our hospital were collected.RT-qPCR was used to detect the relative expression of miR-24-3p in osteosarcoma and para-cancer tissues.MG-63 cells were transfected with miR-24-3p mimics or miR-24-3p inhibitor,and RT-qPCR detected the interference and overexpression efficiency.The proliferation of osteosarcoma cells was evaluated by CCK-8 and EdU assays,and the migration and invasion were assessed by scratch assay and Transwell assay.The expression of PCNA protein was detected by Western blot.Cell apoptosis was detected by flow cytometry.Results:The expression of miR-24-3p in osteosarcoma tissues and cells was significantly down-regulated by RT-qPCR.The proliferation of osteosarcoma cells was increased by CCK-8 and EdU assays when the miR-24-3p expression was down-regulated.The migration and invasion ability of osteosarcoma cells was increased by scratch and Transwell assay,while cell apoptosis was decreased by flow cytometry (P＜0.001).The proliferation of osteosarcoma cells was inhibited by CCK-8 and EdU experiments when the miR-24-3p expression was up-regulated.The migration and invasion of osteosarcoma cells were inhibited by scratch and Transwell experiments after miR-24-3p overexpression,while cell apoptosis was increased (P＜0.01).Western blot results showed that the expression of PCNA protein in the miR-24-3p mimics group was decreased,while that in the miR-24-3p inhibitor group was increased.Conclusion:miR-24-3p is downregulated in osteosarcoma tissues and cells and could inhibit the proliferation,migration and invasion of osteosarcoma cells and promote cell apoptosis.miR-24-3p plays a role in osteosarcoma inhibition and is expected to be one of the therapeutic targets for osteosarcoma patients.

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Memo

Memo:
宁夏回族自治区人民医院培育振兴科研项目（编号：202110）；宁夏医科大学校级科研项目（编号：XM2021080）
Last Update: 1900-01-01