|Table of Contents|

Study on the targeting of the B subunit of calmodulin phosphatase in liver cancer

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 18
Page:
3342-3346
Research Field:
Publishing date:

Info

Title:
Study on the targeting of the B subunit of calmodulin phosphatase in liver cancer
Author(s):
HAN Lifang; TIAN Shuhong
Hainan Drug Safety Evaluation Research Center,Hainan Medical College,Hainan Haikou 571199,China.
Keywords:
hepatocellular carcinoma in situ; calmodulin phosphatase B subunit; tumor targeting; in vivo imaging system
PACS:
R735.7
DOI:
10.3969/j.issn.1672-4992.2023.18.002
Abstract:
Objective:To investigate the targeting of recombinant human calmodulin phosphatase B subunit (rhCNB) in liver cancer based on small animal imaging.Methods：The in situ liver cancer model of BALB/c nude mouse with human hepatoma cell SMMC-7721 was established by tumor tissue block method.On the 14th day after the establishment of the in-situ liver cancer model,the animals were randomly divided into FITC-labeled rhCNB (rhCNB-FITC) administration group and FITC control group according to their body weight.There were 12 tumorous mice in each group,and 6 mice were dissected 2 h and 24 h after administration to remove the heart,lung,liver,stomach,bilateral kidney and spleen for fluorescence detection by small animal imaging system.18 normal BALB/c nude mice without tumor inoculation were randomly divided into rhCNB-FITC administration group and blank control group.Among them,6 blank control group were dissected and the same organs were taken out for fluorescence detection.rhCNB-FITC administration group of 12,6 of the same organs were dissected at 2 h and 24 h after administration for fluorescence detection.Results：rhCNB-FITC was mainly distributed in the kidney,liver,stomach,heart and lung of normal animals without tumor inoculation for 2 h,but not in the spleen.At 24 h,it was mainly distributed in the kidney,but not in the liver,stomach,heart,lung and spleen.In the in-situ liver cancer model test,rhCNB-FITC was mainly distributed in liver tumor,kidney,lung,stomach,heart and other tissues and organs after 2 h administration,and no obvious fluorescence was detected in the spleen.Fluorescence was detected only in liver and stomach tissues in FITC administration group,and the fluorescence intensity was significantly lower than rhCNB-FITC administration group.The comparison of fluorescence intensity of liver (including normal tissue and liver tumor tissue) between rhCNB-FITC administration group and FITC control group was statistically significant (P＜0.01).At 24 h after administration,rhCNB-FITC mainly concentrated in liver tumor,stomach,kidney,lung and other tissues and organs,and the concentration of rhCNB was the highest in liver tumor.The fluorescence intensity of liver tumor in rhCNB-FITC administration group and FITC control group was compared,P＜0.05,which had statistical significance.Conclusion：Recombinant human calmodulin phosphatase B subunit has good targeting properties for liver cancer.

References:

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Memo:
海南省自然科学基金资助项目(编号：818MS068)
Last Update: 1900-01-01