|Table of Contents|

Experimental study on ultrasound-guided vacuum-assisted biopsy and microwave ablation of rabbit breast tumors

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 17
Page:
3191-3197
Research Field:
Publishing date:

Info

Title:
Experimental study on ultrasound-guided vacuum-assisted biopsy and microwave ablation of rabbit breast tumors
Author(s):
FENG Yuran; WEI Lin; LI Xing; ZHAN Jinqin; ZHU Mei
Department of Ultrasound，the First Affiliated Hospital of Kunming Medical University,Yunnan Kunming 650032，China.
Keywords:
ultrasound-guided; vacuum-assisted biopsy; microwave ablation; breast cancer; VX2 tumor
PACS:
R737.9
DOI:
10.3969/j.issn.1672-4992.2023.17.009
Abstract:
Objective:To compare the effect and healing of vacuum-assisted biopsy(VAB) and microwave ablation(MWA) in the treatment of breast cancer in rabbit.Methods:This study built 72 VX2 breast cancer models in rabbits and the rabbits received operation with VAB and MWA randomly,which were 36 in each group.General observation,ultrasound and pathological examination were used for evaluate of the treatments at 1st day,3rd day,7th day,14th day,30th day,60th day after the VAB and MWA.Immunohistochemical detection in CD31,VEGF,TGF-β was used to quantify and evaluate the efficacy and local healing.Results:The success rate of VAB after 30 days was 75.0%,and that of MWA was 83.3%.The incidence of postoperative hematoma in VAB was 13.9%,which would gradually reduce with time.The incidence of postoperative scald in MWA was 13.9%.The granulation tissue reached the peak at 7th day in both group.Then the granulation tissue decreased and had gradually epithelialization,but the time of epithelialization in MWA was later.Quantification indicated that VAB was higer than MWA at 14th day,30th day,60th day.The CD31 expression rate of VAB was higher at 3rd day.The expression level of VEGF in MWA was higher at 14th day and 30th day,and the expression of TGF-β decreased more slowly in MWA.Conclusion:VAB and MWA are both effective and safe methods for VX2 breast cancer in rabbits.VAB may be better than MWA in early healing,and scar formation is not as obvious as MWA.

References:

［1］陈伟,纪晓惠,韩若凌,等.超声引导微波消融治疗兔T1期乳腺癌的可行性研究［J］.中国超声医学杂志,2015,31(5):446-448. CHEN W,JI XH,HAN RL,et al.Research on the feasibility of ultrasound-guided microwave ablation on T1 stage VX2 breast cancer［J］.Chinese J Ultrasound Med,2015,31(5):446-448.
［2］GREENHALGH DG,SPRUGEL KH,MURRAY MJ,et al.PDGF and FGF stimulate wound healing in the genetically diabetic mouse［J］.The American Journal of Pathology,1990,136(6):1235.
［3］ARNOLD M,MORGAN E,RUMGAY H,et al.Current and future burden of breast cancer:Global statistics for 2020 and 2040［J］.Breast,2022,66(9):15-23.
［4］RUGGIRELLO I,NORI J,DESIDERI I,et al.Stereotactic vacuum-assisted breast biopsy:Comparison between 11 and 8-gauge needles［J］.Eur J Surg Oncol,2017,43(12):2257-2260.
［5］PARK HL,KIM KY,PARK JS,et al.Clinicopathological analysis of ultrasound-guided vacuum-assisted breast biopsy for the diagnosis and treatment of breast disease［J］.Anticancer Res,2018,38(4):2455-2462.
［6］GIANNOTTI E,JAMES JJ,CHEN Y,et al.Effectiveness of percutaneous vacuum-assisted excision(VAE) of breast lesions of uncertain malignant potential(B3 lesions) as an alternative to open surgical biopsy［J］.Eur Radiol,2021,31(12):9540-9547.
［7］DUMOLARD L,GHELFI J,ROTH G,et al.Percutaneous ablation-induced immunomodulation in hepatocellular carcinoma［J］.Int J Mol Sci,2020,21(12):4398.
［8］ZHANG J,LI H,LIN L,et al.Ultrasound-guided microwave ablation for multiple benign breast lesions:A prospective study［J］.J Obstet Gynaecol Res,2021,47(9):3362-3369.
［9］PAN H,QIAN M,CHEN H,et al.Precision breast-conserving surgery with microwave ablation guidance:A pilot single-center,prospective cohort study［J］.Front Oncol,2021,11(5):680091.
［10］SHUKLA A,CHOUDURY S,CHAUDHARY G,et al.Chitosan and gelatin biopolymer supplemented with mesenchymal stem cells(Velgraft) enhanced wound healing in goats(Capra hircus):Involvement of VEGF,TGF and CD31［J］.J Tissue Viability,2021,30(1):59-66.
［11］ZHANG L,LIU L,ZHANG J,et al.Porcine fibrin sealant promotes skin wound healing in rats［J］.Evid Based Complement Alternat Med,2022,2022:5063625.
［12］TANG X,YANG Y,YUAN H,et al.Novel transcriptional regulation of VEGF in inflammatory processes［J］.J Cell Mol Med,2013,17(3):386-397.
［13］CAO PF,XU YB,TANG JM,et al.HOXA9 regulates angiogenesis in human hypertrophic scars:induction of VEGF secretion by epidermal stem cells［J］.Int J Clin Exp Pathol,2014,7(6):2998-3007.
［14］KIM YS,LEW DH,TARK KC,et al.Effect of recombinant human epidermal growth factor against cutaneous scar formation in murine full-thickness wound healing［J］.J Korean Med Sci,2010,25(4):589-596.
［15］PAKYARI M,FARROKHI A,MAHARLOOEI MK,et al.Critical role of transforming growth factor beta in different phases of wound healing［J］.Adv Wound Care(New Rochelle),2013,2(5):215-224.

Memo

Memo:
云南省卫生科技计划项目（编号：2017NS039）；云南省医学领军人才项目（编号：L-2019032）；云南省基础研究计划重点项目（编号：202201AS070077）；云南省基础研究计划（编号：202201AY070001-079）
Last Update: 2023-07-31