|Table of Contents|

lncRNA C5orf66-AS1 promotes the proliferation and migration of cervical cancer cells and its mechanism

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 17
Page:
3182-3190
Research Field:
Publishing date:

Info

Title:
lncRNA C5orf66-AS1 promotes the proliferation and migration of cervical cancer cells and its mechanism
Author(s):
BAO Lili; ZHAO Da; YU Yan; ZHOU Qiaomiao
Department of Gynecology,Hainan Women and Children's Medical Center,Hainan Haikou 570100,China.
Keywords:
cervical cancer; lncRNA C5orf66-AS1; miRNA; tumor metastasis
PACS:
R737.33
DOI:
10.3969/j.issn.1672-4992.2023.17.008
Abstract:
Objective:To investigate the effect of lncRNA C5orf66-AS1 on the proliferation and migration of cervical cancer cells and its mechanism.Methods:Differential expression of lncRNA in cervical cancer and adjacent tissues was screened by TCGA,and candidate lncRNA was verified by qRT-PCR.The expression levels of miR-149-5p and RING1 in cervical cancer tissues and cells were detected by qRT-PCR.The effects of C5orf66-AS1 on cell proliferation, migration and apoptosis were detected by CCK-8,clone formation,Transwell and flow cytometry.The regulatory effect of C5orf66-AS1 on the expression of miR-149-5p and RING1 was evaluated by double luciferase assay and Western blot.Furthermore,a nude mouse tumorigenic model was constructed to verify the effect of C5orf66-AS1 on tumor growth by tumor weight and volume.Results:lncRNA C5orf66-AS1 was significantly up-regulated in cervical cancer tissues and cells(P＜0.05).Compared with NC group,C5orf66-AS1 group can promote the proliferation and migration of cervical cancer cells(P＜0.05).The results of RNA immunoprecipitation(RIP) and double luciferase report showed that C5orf66-AS1 bound to miR-149-5p.Overexpression of miR-149-5p and RING1 can reverse the proliferation and migration of cervical cancer cells mediated by C5orf66-AS1.Finally,in vivo experiments in nude mice showed that,compared with NC group,the tumors in C5orf66-AS1 group grew significantly(P＜0.05).Conclusion:As a competitive endogenous RNA,lncRNA C5orf66-AS1 regulates the effect of RING1 on the proliferation,apoptosis and migration of cervical cancer cells by adsorbing miR-149-5p.

References:

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Memo

Memo:
海南省卫生健康行业科研项目（编号：22A200105）
Last Update: 2023-07-31