|Table of Contents|

Effects of interfering Plexin-B1 on autophagy and cisplatin sensitivity of ovarian cancer SKOV3 cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 17
Page:
3166-3170
Research Field:
Publishing date:

Info

Title:
Effects of interfering Plexin-B1 on autophagy and cisplatin sensitivity of ovarian cancer SKOV3 cells
Author(s):
HE Pei; LI Hui; WANG Zhongxian
Department of Gynecology,Wuhan No.1 Hospital,Hubei Wuhan 430022,China.
Keywords:
Plexin-B1; ovarian cancer; autophagy; sensitivity
PACS:
R737.31
DOI:
10.3969/j.issn.1672-4992.2023.17.005
Abstract:
Objective:To investigate the effects of Plexin-B1 interference on autophagy and cisplatin sensitivity of ovarian cancer SKOV3 cells.Methods:Plexin-B1 siRNA sequence and NC sequence were synthesized and transfected into ovarian cancer SKOV3 cells,and the transfection efficiency was detected by qRT-PCR.The cells were divided into control group,NC group and si-Plexin-B1 group.The cell activity was detected by CCK8,and the expression of Plexin-B1 mRNA was detected by qRT-PCR.The protein expressions of Plexin-B1,Akt,p-Akt,LC3-Ⅱ,CALR and PRKDC were detected by Western blot.Cells were treated with different concentrations of cisplatin(1,2,4,8,16,32 μg/mL),the proliferation of cells in each group was detected by CCK8,and the effect of interfering Plexin-B1 on cisplatin sensitivity was evaluated.Results:Compared with the control group,the activity of ovarian cancer SKOV3 cells,the expression levels of Plexin-B1 mRNA and protein,and the phosphorylation level of Akt protein were significantly decreased after Plexin-B1 interference(P＜0.05),the protein expression levels of LC3-Ⅱ,CALR and PRKDC were significantly increased(P＜0.05).CCK8 results showed that the proliferation ability of ovarian cancer SKOV3 cells gradually decreased with the increase of cisplatin concentration,and the proliferation ability of ovarian cancer SKOV3 cells after Plexin-B1 interference was lower than that of the control group,that is,the sensitivity of ovarian cancer SKOV3 cells to cisplatin increased after Plexin-B1 interference.Conclusion:Plexin-B1 can promote the autophagy of ovarian cancer SKOV3 cells through PI3K/Akt pathway,and thus improve the sensitivity of ovarian cancer SKOV3 cells to cisplatin.

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湖北省科技厅科技创新专项立项项目（编号：2021CFB576）
Last Update: 2023-07-31