|Table of Contents|

Aspirin inhibits proliferation and migration of renal carcinoma cells and induces apoptosis and autophagy

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 17
Page:
3145-3153
Research Field:
Publishing date:

Info

Title:
Aspirin inhibits proliferation and migration of renal carcinoma cells and induces apoptosis and autophagy
Author(s):
JIA Wei1; SHI Yu1; MA Yanjie2; ZHENG Jinxiu1; LIU Shenglu1; SUO Limin3; YANG Tao1
1.Department of Biochemistry and Molecular Biology,School of Basic Medicine,Shanxi Medical University,Shanxi Jinzhong 030600,China;2.Department of Urology,the First Clinical Medical College of Shanxi Medical University,Shanxi Taiyuan 030000,China；3.Department of Otolaryngology Head and Neck Surgery,the Second Hospital of Shanxi Medical University,Shanxi Taiyuan 030000,China.
Keywords:
aspirin; kidney cancer; autophagy; proliferation; migration
PACS:
R737.11
DOI:
10.3969/j.issn.1672-4992.2023.17.002
Abstract:
Objective:To investigate the effects of aspirin(acetyl salicylic acid，ASA) on the proliferation,migration,apoptosis and autophagy of human renal carcinoma cell lines ACHN and 786-O.Methods:CCK-8 method was used to determine the effect of different concentrations of ASA and chloroquine(CQ) combined with ASA on the survival rate of ACHN and 786-O cells,and plate cloning experiment was used to elucidate the effect of different concentrations of ASA on the clonogenesis ability of renal cancer cells.The migration ability of renal cancer cells was detected by Transwell assay and scartch assay,apoptosis of renal cancer cells was detected by Annexin V-FITC/PI assay.The effects of ASA on apoptosis related proteins Bax,Bcl-2 and autophagy related proteins LC3B,Beclin-1 and p62 of renal cancer cells were detected by Western blot.Results:ASA could observably inhibit the survival rate of ACHN and 786-O cells,and the survival rate of ACHN and 786-O cells decreased remarkably with the increase of ASA concentration/time(P＜0.05).Compared with the control group,ASA significantly inhibited the proliferation and migration of renal carcinoma cells(P＜0.05).ASA treatment increased the number of apoptosis of renal cancer cells,significantly inhibited the expression of apoptosis-related protein Bcl-2,up-regulated the expression of Bax,promoted the expressions of autophagy related proteins LC3B and Beclin-1,and inhibited the expression of p62(P＜0.05).Compared with CQ treatment group,ASA combined with CQ treatment group significantly promoted the expression of LC3B and inhibited the expression of p62(P＜0.05),and significantly inhibited the proliferation and migration of renal cancer cells and promoted apoptosis(P＜0.05).Conclusion:ASA can inhibit the proliferation and migration of renal carcinoma cells and promote apoptosis and autophagy.

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Memo

Memo:
National Natural Science Foundation of China（No.42107466）;国家自然科学基金（编号：42107466）；山西省基础研究计划（编号：202103021223226）
Last Update: 2023-07-31