|Table of Contents|

Effect of hsa-miR-767 on the biological behavior of breast cancer cells by regulating IGF1 gene

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 16
Page:
3002-3009
Research Field:
Publishing date:

Info

Title:
Effect of hsa-miR-767 on the biological behavior of breast cancer cells by regulating IGF1 gene
Author(s):
ZENG Ru1; MAO Shanshan2
1.Cancer Radiotherapy Department;2.Cancer Chemotherapy Department,Haikou People's Hospital,Hainan Haikou 570208,China.
Keywords:
breast cancer; miRNA-mRNA axis; TOP10 hub gene; hsa-miR-767
PACS:
R737.9
DOI:
10.3969/j.issn.1672-4992.2023.16.010
Abstract:
Objective:To explore the effect of hsa-miR-767 regulating IGF1 on the biological behavior of human breast cancer cells.Methods:The bioinformatics data were used to analyze the differential genes in breast cancer,and the miRNA-mRNA network was constructed to screen hsa-miR-767/IGF1 signal axis for verification.Establish a MCF7 cell line with hsa-miR-767 overexpression and knockout.The expression of hsa-miR-767 was verified by RT-qPCR.The binding effect of hsa-miR-767 and IGF1 by dual luciferase reporter gene assay was detected.CCK-8 experiment,scratch experiment,Transwell experiment and flow cytometry were used to detect the proliferation,migration,invasion and apoptosis of breast cancer cells.Results:26 differential miRNAs (DEMs) and 1 108 differential genes (DEGs) were identified.It was predicted that 254 target genes were obtained,and 73 mRNAs were obtained by crossing the target genes with sequencing DEGs.10 hub genes (IGF1,NTRK2,CEBPA,FOS,KLF4,GABRA1,NRG1,LPL,GABBR2,ANGPT1) were identified,among which IGF1 was linked to many genes,indicating that it was more important in breast cancer.The hsa-miR-767/IGF1 axis found in the miRNA-mRNA network may play a key role in breast cancer.Cell experiment confirmed that expression level of hsa-miR-767 was up-regulated in breast cancer (P＜0.05),and it could target IGF1.hsa-miR-767 can also promote the proliferation,migration and invasion of breast cancer cells,and inhibit cell apoptosis (P＜0.05).Conclusion:hsa-miR-767 can promote the proliferation,migration and invasion of breast cancer cells,inhibit apoptosis,and bind to IGF1.

References:

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