|Table of Contents|

Bioinformatic analysis and experimental verification of prognostic miRNA in oral squamous cell carcinoma

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 16
Page:
2988-2995
Research Field:
Publishing date:

Info

Title:
Bioinformatic analysis and experimental verification of prognostic miRNA in oral squamous cell carcinoma
Author(s):
GOU Qiutong<sup>1; 2; 3</sup>; XIE Qiang<sup>4</sup>; JIN Wenke<sup>5</sup>; LIAO Minru<sup>4</sup>; LI Zixiang<sup>5</sup>; HUANG Haixia<sup>1; 2; 3</sup>
Keywords:
oral squamous cell carcinoma; miRNA; bioinformatics; prognosis; biomarkers; risk model; PIK3CB
PACS:
R739.8
DOI:
10.3969/j.issn.1672-4992.2023.16.008
Abstract:
Objective:To screen out miRNAs significantly associated with the prognosis of oral squamous cell carcinoma (OSCC) using bioinformatics analysis,and validate it through in vitro experiments.Methods:OSCC data were downloaded from The Cancer Genome Atlas (TCGA).Differentially expressed miRNAs were analyzed by R.The risk model was established using regression analysis.The prognosis of OSCC patients was predicted using Kaplan-Meier survival analysis.The reliability of the risk model was verified by receiver operating characteristic (ROC) curve and regression analysis.Biological functions of prognostic miRNAs were predicted by GO and KEGG analyses.In vitro validation of bioinformatics results was conducted through qRT-PCR and Western blot.Results:Twenty differentially expressed miRNAs in OSCC were screened out.miR-204-5p and miR-503-3p were significantly correlated with the prognosis of OSCC.The risk model constructed in this study could reliably predict the prognosis of OSCC.GO analysis showed miR-204-5p was related to cell apoptosis,proliferation and migration,while miR-503-3p was related to cell differentiation,cell cycle and GTP binding.KEGG analysis showed miR-204-5p was related to PI3K/Akt/mTOR pathway and autophagy pathway,while miR-503-3p was related to Ras pathway and HIF-1 pathway.qRT-PCR showed that the expression of miR-204-5p in OSCC cells was decreased (P＜0.05),while miR-204-5p level in OSCC cells was artificially increased after transient transfection of miR-204-5p mimic (P＜0.05).In addition,Western blot showed that the expression of PIK3CB in OSCC cells was decreased after miR-204-5p elevation (P＜0.05).Conclusion:The results showed that miR-204-5p and miR-503-3p were effective prognostic biomarkers in OSCC.The risk model constructed by miR-204-5p and miR-503-3p could effectively predict the prognosis of OSCC patients.The expression of miR-204-5p in OSCC cells was low.After elevation of miR-204-5p,the expression of PIK3CB in OSCC cells was decreased.

References:

[1] ALMANGUSH A,MKITIE AA,TRIANTAFYLLOU A,et al.Staging and grading of oral squamous cell carcinoma:An update［J］.Oral Oncology,2020,107:104799.
<br/>[2] JOSEPH JP,HARISHANKAR MK,PILLAI AA,et al.Hypoxia induced EMT:A review on the mechanism of tumor progression and metastasis in OSCC［J］.Oral Oncology,2018,80:23-32.
<br/>[3] FANG T,LV H,LV G,et al.Tumor-derived exosomal miR-1247-3p induces cancer-associated fibroblast activation to foster lung metastasis of liver cancer［J］.Nature Communications,2018,9(1):191.
<br/>[4] PAN G,LIU Y,SHANG L,et al.EMT-associated microRNAs and their roles in cancer stemness and drug resistance［J］.Cancer Communications (London,England),2021,41(3):199-217.
<br/>[5] SUN S,WANG J,LIU J,et al.MiR-302b suppresses tumor metastasis by targeting frizzled 6 in OSCC［J］.Journal of Dental Research,2021,100(7):739-745.
<br/>[6] RIEMANN M,KNIPFER C,ROHDE M,et al.Oral squamous cell carcinoma of the tongue:Prospective and objective speech evaluation of patients undergoing surgical therapy［J］.Head & Neck,2016,38(7):993-1001.
<br/>[7] MORATIN J,HORN D,METZGER K,et al.Squamous cell carcinoma of the mandible - Patterns of metastasis and disease recurrence in dependence of localization and therapy［J］.Journal of Cranio-maxillo-facial Surgery,2020,48(12):1158-1163.
<br/>[8] GOETZ C,RASCHKA J,WOLFF KD,et al.Hospital based quality of life in oral cancer surgery［J］.Cancers,2020,12(8):2152.
<br/>[9] LI X,GUO S,XIONG XK,et al.Combination of quercetin and cisplatin enhances apoptosis in OSCC cells by downregulating xIAP through the NF-κB pathway［J］.Journal of Cancer,2019,10(19):4509-4521.
<br/>[10] FENG X,LUO Q,ZHANG H,et al.The role of NLRP3 inflammasome in 5-fluorouracil resistance of oral squamous cell carcinoma［J］.Journal of Experimental & Clinical Cancer Research,2017,36(1):81.
<br/>[11] HILL M,TRAN N.miRNA interplay:mechanisms and consequences in cancer［J］.Disease Models & Mechanisms,2021,14(4):dmm047662.
<br/>[12] HAN TS,HUR K,CHO HS,et al.Epigenetic associations between lncRNA/circRNA and miRNA in hepatocellular carcinoma［J］.Cancers,2020,12(9):2622.
<br/>[13] LIU Z,LIU Q,WANG X,et al.Multiomics immune-related lncRNA analysis of oral squamous cell carcinoma and its correlation with prognosis［J］.Disease Markers,2022,2022:6106503.
<br/>[14] YAO Y,XU Q,YAN L,et al.MiRNA-128 and miRNA-142 regulate tumorigenesis and EMT in oral squamous cell carcinoma through HOXA10［J］.Cancer Management and Research,2020,12:9987-9997.
<br/>[15] WANG J,SONG R,WANG C,et al.miR-195 inhibits proliferation and enhances apoptosis of OSCC cells via targeting TLR4［J］.Journal of Healthcare Engineering,2022,2022:2270777.
<br/>[16] ALI AHMED E,ABD EL-BASIT SA,MOHAMED MA,et al.Clinical role of miRNA 29a and miRNA 335 on breast cancer management:their relevance to MMP2 protein level［J］.Archives of Physiology and Biochemistry,2022,128(4):1058-1065.
<br/>[17] LIANG G,MENG W,HUANG X,et al.miR-196b-5p-mediated downregulation of TSPAN12 and GATA6 promotes tumor progression in non-small cell lung cancer［J］.Proceedings of the National Academy of Sciences of the United States of America,2020,117(8):4347-4357.
<br/>[18] YANG S,CHEN B,ZHANG B,et al.miR-204-5p promotes apoptosis and inhibits migration of gastric cancer cells by targeting HER-2［J］.Molecular Medicine Reports,2020,22(4):2645-2654.
<br/>[19] SU Q,SHEN H,GU B,et al.miR-204-5p hampers breast cancer malignancy and affects the cell cycle by targeting PRR11［J］.Computational and Mathematical Methods in Medicine,2022,2022:4010947.
<br/>[20] LUO H,LV W,ZHANG H,et al.miR-204-5p inhibits cell proliferation and induces cell apoptosis in esophageal squamous cell carcinoma by regulating Nestin［J］.International Journal of Medical Sciences,2022,19(3):472-483.
<br/>[21] YANG H,LI Z,WANG Z,et al.Histocompatibility minor 13 (HM13),targeted by miR-760,exerts oncogenic role in breast cancer by suppressing autophagy and activating PI3K-AKT-mTOR pathway［J］.Cell Death & Disease,2022,13(8):728.
<br/>[22] LIAO S,LIANG L,YUE C,et al.CD38 is involved in cell energy metabolism via activating the PI3K/AKT/mTOR signaling pathway in cervical cancer cells［J］.International Journal of Oncology,2020,57(1):338-354.
<br/>[23] YANG J,NIE J,MA X,et al.Targeting PI3K in cancer:mechanisms and advances in clinical trials［J］.Molecular Cancer,2019,18(1):26.
<br/>[24] MAZLOUMI GAVGANI F,SMITH ARNESEN V,JACOBSEN RG,et al.Class I phosphoinositide 3-kinase PIK3CA/p110α and PIK3CB/p110β isoforms in endometrial cancer［J］.International Journal of Molecular Sciences,2018,19(12):3931.
<br/>[25] XU W,WANG Z,ZHANG Z,et al.PIK3CB promotes oesophageal cancer proliferation through the PI3K/AKT/mTOR signalling axis［J］.Cell Biology International,2022,46(9):1399-1408.
<br/>[26] ZHANG Z,ZHANG L,WANG B,et al.MiR-337-3p suppresses proliferation of epithelial ovarian cancer by targeting PIK3CA and PIK3CB［J］.Cancer Letters,2020,469:54-67.

Memo

Memo:
四川省泸州市科技计划项目(编号：2021-JYJ-68)；西南医科大学校级科研面上项目(编号：2021ZKMS014)
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