|Table of Contents|

C1ql4 regulates the characteristics of breast cancer stem cells and epithelial-mesenchymal transition through PI3K/Akt signaling pathway

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 16
Page:
2977-2982
Research Field:
Publishing date:

Info

Title:
C1ql4 regulates the characteristics of breast cancer stem cells and epithelial-mesenchymal transition through PI3K/Akt signaling pathway
Author(s):
XU Fan; ZHANG Qing; LI Xiao
Department of Oncology,Affiliated Hospital of Chengde Medical University,Hebei Chengde 067000,China.
Keywords:
breast cancer; C1ql4; migration; epithelial-mesenchymal transition; apoptosis; PI3K/Akt
PACS:
R737.9
DOI:
10.3969/j.issn.1672-4992.2023.16.006
Abstract:
Objective:To explore the effects of C1ql4 on the characteristics of breast cancer stem cells and the epithelial mesenchymal transition.Methods:siRNA interference technique was used to down-regulate the expression of C1ql4 in BT549 cell line.RT-qPCR and Western blot methods were used to verify the silence of C1ql4.The subsequent experiments were divided into control group,empty vector group(si-NC group) and C1ql4 silence group(si-C1ql4 group).The mRNA and protein expression of stem cell characteristic-related protein(CD44,Nanog,OCT4) and EMT-related proteins(E-cadherin,Vimentin,N-cadherin) in each group were detected,respectively.The apoptosis,CD44+CD24-/low cell content of cells in each group were detected by flow cytometry,and the spheroidizing ability of cells in each group was compared by spheroidizing test,and the migration ability of cells in each group was compared by Transwell chamber test.Western blot was used to detect the expression level of signal pathway proteins,and explore the possible mechanism of C1ql4 affecting the characteristics of breast cancer stem cells and epithelial mesenchymal transformation.Results:After down-regulation of C1ql4 expression,the mRNA and protein expression of CD44,Nanog,OCT4,Vimentin and N-cadherin,breast cancer stem cell ratio,balling and migration ability of si-C1ql4 group were lower than those of control group and si-NC group,while the expression of E-cadherin mRNA and protein,apoptosis rate were significantly higher than those of the other two groups.The protein expression level and phosphorylation level of PI3K and Akt were also significantly lower than those of control group and si-NC group(P＜0.05),but there was no significant difference between control group and si-NC group.Conclusion:The expression of C1ql4 can promote the expression of breast cancer stem cells and the epithelial mesenchymal transformation,enhance the biological behavior of breast cancer cells such as spheroidization and migration,inhibit cell apoptosis.The mechanism of action may be related to PI3K/Akt signaling pathway.

References:

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Memo

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河北省自然科学基金项目(编号：H2021406036)；河北省承德市应用技术研究与开发暨可持续发展议程创新示范区专项科技计划项目（编号：202205B073）
Last Update: 1900-01-01