|Table of Contents|

Relationship between NFX1-123 and HPV16 E6 in HPV-positive cervical cancer cells and its possible mechanism

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 16
Page:
2972-2976
Research Field:
Publishing date:

Info

Title:
Relationship between NFX1-123 and HPV16 E6 in HPV-positive cervical cancer cells and its possible mechanism
Author(s):
LI Jiaosheng1; 2; GUO Yifan2; 3; YU Haotian1; 3
1.The Second School of Clinical Medicine,Southern Medical University,Guangdong Guangzhou 510168,China;2.Department of Obstetrics and Gynecology;3Reproductive Medicine Center,Hainan Hospital of PLA General Hospital,Hainan Sanya 572013,China.
Keywords:
NFX1-123; cervical cancer; high risk-human papilloma virus; E6
PACS:
R737.33
DOI:
10.3969/j.issn.1672-4992.2023.16.005
Abstract:
Objective:To construct and verify the eukaryotic expression plasmid of NFX1-123 gene,and to explore its cell localization relationship with HPV16 E6 in HPV positive cervical cancer cells and its possible mechanism.Methods:NFX1-123 gene fragment was obtained by PCR and inserted into the nHA/pRK5 vector.Restriction enzyme digestion and sequencing were used to identified the NFX1-123 recombinant plasmid.Western blot was used to confirm the overexpression of NFX1-123.Co-immunoprecipitation was used to certify its interaction with HPV16 E6.Results:The result of enzyme digestion and sequencing showed that NFX1-123 was successfully cloned into nHA/pRK5.Western blot showed that HA-NFX1-123 protein could be normally expressed in HEK-293T cells.The immunofluorescence revealed that NFX1-123 was mainly localized in the cytoplasm.The interaction between NFX1-123 and HPV16 E6 was demonstrated by co-immunoprecipitation.When HPV16 E6 was co-expressed,NFX1-123 underwent a change in localization from the cytoplasm to the nucleus and co-localized with HPV16 E6 in cell nucleus.Conclusion:Eukaryotic nHA-NFX1-123/pRK5 was successfully constructed.The interaction between NFX1-123 and HPV16 E6 was confirmed.HPV16 E6 could affect the subcellular localization of NFX1-123,shifting it from the cytoplasm to the nucleus.Supplementary explanation was provided on why NFX1-123 in cytoplasm can bind to HPV16 E6 in the nucleus and promote HR-HPV infected cervical epithelium to cervical cancer through various pathways.

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海南省自然科学基金面上项目（编号：820MS127）
Last Update: 1900-01-01