|Table of Contents|

ARF4 regulates the proliferation,migration,invasion and apoptosis of non-small cell lung cancer cells through EGFR pathway

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 16
Page:
2965-2971
Research Field:
Publishing date:

Info

Title:
ARF4 regulates the proliferation,migration,invasion and apoptosis of non-small cell lung cancer cells through EGFR pathway
Author(s):
PEI Yao; XU Lihuan; HU Yaorui; HUANG Tingting; YANG Chunhua; TIAN Geng
Shandong Technology Innovation Center of Molecular Targeting and Intelligent Diagnosis and Treatment,Binzhou Medical University,Shandong Yantai 264003,China.
Keywords:
ARF4; NSCLC; cisplatin; proliferation; apoptosis
PACS:
R734.2
DOI:
10.3969/j.issn.1672-4992.2023.16.004
Abstract:
Objective:To investigate the effects and mechanisms of ADP-ribosylation factor 4(ARF4) in the development of non-small cell lung cancer（NSCLC).Methods:The expression level of ARF4 in clinical NSCLC tissues and paracarcinoma tissues was analyzed by Western blot（WB) and immunohistochemistry.The correlation of ARF4 and patient prognosis with NSCLC was verified based on TCGA database.The expression level of ARF4 in human NSCLC cell lines A549 and H1975 cells was knocked down by siARF4 and knockdown efficiency was detected by WB and real-time quantitative PCR(RT-qPCR) analysis.The effect of ARF4 knockdown or combination of ARF4 knockdown and cisplatin treatment on the proliferation of NSCLC cells was detected by CCK-8 assay.The effect of ARF4 knockdown or combination of ARF4 knockdown and cisplatin treatment on migration and invasion of NSCLC cells was verified by Transwell assay.The effect of ARF4 knockdown or combination of ARF4 knockdown and cisplatin treatment on apoptosis of NSCLC was detected by flow cytometry analysis.The effect of ARF4 knockdown on EGFR pathways in NSCLC was determined by WB analysis.Results:The expression of ARF4 in NSCLC tissues was significantly higher than that in paracarcinoma tissues,and was significantly correlated with poor prognosis of patients.Knockdown of ARF4 in A549 and H1975 cells inhibited cell proliferation,migration,invasion and promoted cell apoptosis.The combination of ARF4 knockdown and cisplatin treatment could further inhibit cell proliferation,migration,invasion,and increase cell apoptosis.The knockdown of ARF4 inhibited the activation of EGFR pathways in NSCLC cells.Conclusion:ARF4 may regulate the physiological function of NSCLC through EGFR pathways.The combination of ARF4 knockdown and cisplatin effectively inhibited cell proliferation,migration,invasion,and promoted cell apoptosis.

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Memo:
山东省自然科学基金（编号：ZR2021MH141）
Last Update: 1900-01-01