|Table of Contents|

HER-2 enhances the invasion,migration and adhesion ability of gastric cancer cells by affecting EMT

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 16
Page:
2956-2960
Research Field:
Publishing date:

Info

Title:
HER-2 enhances the invasion,migration and adhesion ability of gastric cancer cells by affecting EMT
Author(s):
ZHANG Xue1; ZHAO Lili2; FENG Qingqing3; ZHAO Wenwen3; ZHAO Wenfei3; JING Wenjun3; YANG Shuai3; YU Xiaopeng3; WEI Hongmei3
1.Medical College of Qingdao University,Shandong Qingdao 266042，China;2.Qingdao Traditional Chinese Medicine Hospital(Qingdao Hiser Hospital)，Qingdao Hiser Hospital Affiliated of Qingdao University,Shandong Qingdao 266042,China；3.Affiliated Qingdao Central Hospital of Qingdao University,Qingdao Cancer Hospital,Shandong Qingdao 266042,China.
Keywords:
gastric malignancies; HER-2; invasion; migration; adhesion; EMT
PACS:
R735.2
DOI:
10.3969/j.issn.1672-4992.2023.16.002
Abstract:
Objective:To study the effect of HER-2 expression on gastric cancer cell invasion,migration and adhesion,and to investigate the relationship between HER-2 expression and EMT related protein.Methods:HER-2 expression was silenced in MKN-45 gastric cancer cells by small interfering RNA.The silencing of HER-2 was verified by RT-PCR and Western blot,and the experiment was divided into blank control,negative control and HER-2 silencing groups.The mRNA and protein expression of E-cadherin,Vimentin and Snail（EMT related protein) were detected by RT-PCR and Western blot.Cell invasion and migration were measured by Transwell chamber technique.Cell adhesion were measured by MTT experiment.Results:After silencing of HER-2 expression in MKN-45 gastric cancer cell lines,Vimentin,Snail and adhesion of HER-2 silencing group were significantly lower than the blank control and negative control group.E-cadherin in HER-2 silencing group was significantly higher than the blank control and negative control group,and the differences were statistically significant（P＜0.05).Conclusion:In gastric cancer cells,HER-2 may enhance the invasion,migration and adhesion ability of gastric cancer cells by affecting the EMT related proteins.

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Memo

Memo:
山东省青岛市医药卫生科研计划项目（编号：2021-WJZD081）
Last Update: 1900-01-01