|Table of Contents|

Expression of GRP78 in cholangiocarcinoma tissues and its relationship with prognosis

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 15
Page:
2861-2867
Research Field:
Publishing date:

Info

Title:
Expression of GRP78 in cholangiocarcinoma tissues and its relationship with prognosis
Author(s):
GAO Long1; 2; 3; MA Haidong1; 2; 3; BAI Mingzhen1; 2; 3; FU Wenkang1; 2; 3; MI Ningning1; 2; 3; LIN Yanyan1; 2; YUE Ping1; 2; ZHANG Jinduo1; 2; MENG Wenbo1; 2; 3
1.The First Clinical Medical College of Lanzhou University,Gansu Lanzhou 730000,China；2.Department of General Surgery,First Hospital of Lanzhou University,Gansu Lanzhou 730000,China；3.The Key Laboratory of Biological Therapy and Regenerative Medicine,Gansu Province,Gansu Lanzhou 730000,China.
Keywords:
biliary tumor; GRP78; cholangiocarcinoma; prognosis; biomarker
PACS:
R735.8
DOI:
10.3969/j.issn.1672-4992.2023.15.018
Abstract:
Objective:To investigate the expression of GRP78 in cholangiocarcinoma and its prognostic value.Methods:The cancer tissues of patients with cholangiocarcinoma were collected,and differential proteomics was performed using iTRAQ.The expression of GRP78 was detected by qRT-PCR and Western blot.Tissue microarray and immunohistochemistry were used to study the correlation between GRP78 and prognosis.Results:A total of 147 differential proteins were screened by iTRAQ,among which heat shock protein 70 family member HSPA5 (GRP78) was aberrantly highly expressed.The results of Western blot showed that GRP78 protein in cancer tissues was significantly higher than that in adjacent tissues,and the average relative expression of GRP78 mRNA was 2.90 and 0.89 in cancer tissues and adjacent tissues respectively,the difference between them was 3.26 times,t=4.834,P＜0.001.Kaplan-Meier survival curves showed that the median overall survival time and disease-free survival time of patients with high GRP78 were 6.83 and 3.74 months respectively,while in patients with low GRP78 were 12.01 and 11.70 months respectively.Patients with high expression of GRP78 had a lower overall survival rate and were prone to relapse,P＜0.05.Univariate Cox analysis indicated that high expression of GRP78,tumor volume ≥5 cm,intrahepatic cholangiocarcinoma,positive vascular invasion and high TNM stage were closely related to the survival time and disease-free survival time (all P＜0.05).Multivariate Cox analysis found that high expression of GRP78,intrahepatic cholangiocarcinoma and tumor volume ≥5 cm were independent risk factors for recurrence (all P＜0.05),and high expression of GRP78 was an independent risk factor for overall survival (P＜0.05).Conclusion:GRP78 is highly expressed in cholangiocarcinoma.The high expression of GRP78 is closely related to poor prognosis and it is a potential prognostic marker and therapeutic target for cholangiocarcinoma.

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Memo

Memo:
National Natural Science Foundation of China(No.81872036）；国家自然科学基金面上项目（编号：81872036）；甘肃省兰州市城关区科技计划项目（编号：2019RCCX0038，2019JSCX0092）
Last Update: 2023-06-30