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Effects of biological clock gene Per1 on the invasion and migration of nasopharyngeal carcinoma cells CNE2 and the expression of JAK2-STAT3 pathway-related proteins

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 15
Page:
2823-2828
Research Field:
Publishing date:

Info

Title:
Effects of biological clock gene Per1 on the invasion and migration of nasopharyngeal carcinoma cells CNE2 and the expression of JAK2-STAT3 pathway-related proteins
Author(s):
LUO Pan; WU Weili; JIN Feng; LONG Jinhua; LI Yuanyuan; ZENG Yan; WANG Ziqi; YE Yongying; CHEN Yu; ZHANG Mang; TANG Hong
Department of Oncology,Affiliated Cancer Hospital of Guizhou Medical University/Affiliated Hospital of Guizhou Medical University,Guizhou Guiyang 550000,China.
Keywords:
nasopharyngeal carcinoma cell CNE2; biological clock gene Per1; JAK2-STAT3 signaling pathway; invasion and metastasis
PACS:
R739.62
DOI:
10.3969/j.issn.1672-4992.2023.15.011
Abstract:
Objective:To explore the the effects of biological clock gene Per1 on invasion and epithelial-mesenchymal transition(EMT) of nasopharyngeal carcinoma cells CNE2 and its relationship with the expression of JAK2-STAT3 signaling pathway-related proteins.Methods:Per1 gene and protein levels in CNE2 cells were detected by real-time fluorescence quantitative PCR(qPCR) and Western blot(WB).Per1 gene overexpression and interference lentivirus vector was constructed and transfed into CNE2 cells.The experiment was divided into overexpression group(Per1-OE),overexpression control group(Per1-OENC),interference group(Per1-sh),interference control group(Per1-shNC).The ability of Per1 gene to invade and migrate cells in each group was detected by scratch healing and Transwell test.WB was used to detect the effect of Per1 gene on the expression of EMT-related proteins:E-cadherin,N-cadherin and Vimentin.WB detected the effects of Per1 gene on the espression of JAK2-STAT3Y signaling pathway-related proteins.Results:WB and PCR results showed that compared with human immortalized nasopharyngeal epithelial cell NP69,the expression of Per1 in nasopharyngeal carcinoma cell CNE2 was decreased.By constructing Per1 gene overexpression and interfering with lentivirus vector,effectively up-regulates and down-regulates the expression of Per1 in the transcription and protein levels of CNE2,and successfully screened the stable transformation cell lines.The scratch test results showed that the cell migration rate of Per1-OE group was significantly lower than that of Per1-OENC group at 24 h and 48 h(P=0.04,P=0.01),and that of Per1-sh group was significantly higher than that of Per1-shNC group at 24 h and 48 h(P=0.01,P=0.005).The results of Transwell experiment showed that the number of cells passing through Transwell cells in Per1-sh group was significantly higher than that in Per1-shNC group(P=0.02),and the number of cells passing through Transwell cells in Per1-OE group was significantly lower than that in Per1-OENC group(P=0.001).WB results showed that there was no significant difference in the expression of JAK2,p-JAK2 and STAT3 proteins in each group(P＞0.05).The protein expression of p-STAT3,N-cadherin and Vimentin in Per1-sh group was lower than that in Per1-shNC group,and the expression of E-cadherin was higher(P＜0.05).The protein expression of p-STAT3,N-cadherin and Vimentin in Per1-OE group was higher than that in Per1-OENC group,and the expression of E-cadherin was lower(P＜0.05).Conclusion:Overexpression of Per1 gene can inhibit the invasion and migration of nasopharyngeal carcinoma cell CNE2,reduce the expression of N-cadherin,Vimentin,p-STAT3 protein,and increase the expression of E-cadherin.After interfering with Per1 gene,it is the opposite,which indicates that Per1 gene may play the role of tumor suppressor gene in nasopharyngeal carcinoma cell CNE2.We speculate that Per1 gene has a certain correlation with the expression of p-STAT3,but its specific mechanism needs further study.

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Memo

Memo:
National Natural Science Foundation of China（No.82060555）;国家自然科学基金项目（编号：82060555）；贵州省卫生健康委科学技术基金项目（编号：gzwj-2022-022）；贵州医科大学国家自然资金培育项目（编号：19NSP047）
Last Update: 2023-06-30