|Table of Contents|

Effect of Compound Kushen Injection on radiation induced oral ulcer model in rats

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 15
Page:
2803-2809
Research Field:
Publishing date:

Info

Title:
Effect of Compound Kushen Injection on radiation induced oral ulcer model in rats
Author(s):
WANG Wei; ZHOU Yan; WANG Xiaohui; LI Jianwei; QIN Wenjie; HAI Lina; LI Anping; YOU Rongli
Beijing Zhendong Guangming Pharmaceutical Research Institute，Beijing 100085，China.
Keywords:
Compound Kushen Injection; radiation induced oral ulcer; radiotherapy
PACS:
R730.55
DOI:
10.3969/j.issn.1672-4992.2023.15.008
Abstract:
Objective:To construct a rat oral mucositis model to evaluate and compare the therapeutic effects of different administration methods of Compound Kushen Injection(CKI) on radiation oral mucositis.Methods:A total of 90 SPF-grade SD rats that were quarantine-qualified were selected,half of male and female,weighing 178.6～388.3 g,randomly divided into 9 groups according to body weight and gender.They were normal control group,model control group,Jinyin peptide(recombinant human epidermal growth factor) group.CKI intravenous injection group(1.8 mL/kg),CKI spray group,CKI oral dose 1,2,3,4 groups(0.45,0.9,1.8,3.6 mL/kg),10 rats for each group.The mouth and inner oral mucosa of rats were irradiated with X-rays at a dose of 15 Gy,and another 10 normal control groups were taken without treatment.After irradiation,each animal was injected 4 mL/mouse of 0.9% sodium chloride.Animals in each group were given different concentrations of drugs at 24 hours after modeling.General clinical observations were performed on each group of animals every day.Body weight,food intake and oral mucosal scores were regularly monitored.After the last administration,rats in each group were euthaniz ed and oral mucosal tissues were taken.Carry out cytokine IL-6,TNF-α,IL-1β content detection,and oral mucosal tissue for histopathological examination.Results:All oral doses of CKI can alleviate the degree of X-ray-induced oral mucosal lesions in rats.Among them,CKI at the doses of 1.8 and 3.6 mL/kg can significantly increase the growth rate of animal weight and food intake,and reduce oral mucosal score,and can reduce IL-6,IL-1β levels.Oral administration of CKI has a significant protective effect on the oral mucosa of rats caused by X-rays.At the same dose,the CKI oral group 1.8 mL/kg was more effective than CKI intravenous injection group(1.8 mL/kg) in rats.Jinyin peptide and CKI intravenous injection group can significantly increase animal body weight and food intake,reduce oral mucosal scores on the fourth and seventh days and reduce IL-6 and IL-1β level in oral mucosal tissues at the same time.Conclusion:Oral administration of CKI has a significant protective effect on X-ray-induced oral mucositis in rats,and its mechanism of action may be related to reducing the level of inflammatory factors.At the same dose,the effect of oral dose of CKI(1.8 mL/kg) on oral mucositis in rats was better than that of intravenous administration of CKI.

References:

[1]TROTTI A,BELLM LA,EPSTEIN JB,et al.Mucositis incidence,severity and associated outcomes in patients with head and neck cancer receiving radiotherapy with or without chemotherapy:a systematic literature review［J］.Radiotherapy & Oncology,2003,66(3):253-262.
[2]包婺安,胡福军,李斌,等.西妥昔单抗联合放化疗治疗复发或进展期头颈部恶性肿瘤的临床观察［C］.杭州：2011国际暨全国第十一届头颈肿瘤学术大会论文汇编,2011:319-326. BAO WA,HU FJ,LI B,et al.Clinical observation of cetuximab combined with radiotherapy and chemotherapy in the treatment of recurrent or advanced head and neck malignant tumors［C］.Hangzhou:2011 International and National 11th Head and Neck Cancer Congress,2011:319-326.
[3]WEE J.Concurrent chemotherapy-enhanced radiation:Trials and conclusions［M］.Springer Berlin Heidelberg,2010:167-181.
[4]安军海,袁彩云.康复新防治头颈部肿瘤同步放化疗引起的口腔黏膜炎［J］.肿瘤研究与临床,2012,24(11):3. AN JH,YUAN CY.Rehabilitation and prevention of oral mucositis caused by simultaneous radiotherapy of head and neck tumours ［J］.Cancer Research and Clin,2012,24(11):3.
[5]SILANDER E,NYMAN J,HAMMERLID E.An exploration of factors predicting malnutrition in patients with advanced head and neck cancer［J］.Laryngoscope,2013,123(10):2428-2434.
[6]MARIA OM,ELIOPOULOS N,MUANZA T.Radiation-induced oral mucositis［J］.Front Oncol,2017,7:89.
[7]许郑林,王蕾,靳泳秀,等.复发性口腔溃疡与免疫相关因素水平变化的研究进展［J］.医学综述,2021,27(24):4893-4897. XU ZL,WANG L,JIN YX,et al.Research progress in recurrent oral ulcer and level changes of related immune factors［J］.Medical Recapitulate,2021,27(24):4893-4897.
[8]管翠强,郭洪波,武云霞,等.复发性口腔溃疡患者的氧化应激与细胞免疫状态研究［J］.中国药物与临床,2018,18(6):884-885. GUAN CQ,GUO HB,WU YX,et al.Study of oxidative stress and cellular immune status in patients with recurrent oral ulcers［J］.Chinese Remedies & Clinics,2018,18(6):884-885.
[9]王进,王小平,何军.锡类散缓释贴膜对复发性阿弗他溃疡家兔肿瘤坏死因子-α,白细胞介素-1及白细胞介素-2的影响［J］.河北中医,2011,33(9):1381-1383. WANG J,WANG XP,HE J.Effect of Xilei oral sustained-release mucoadhesive films on TNF-α,IL-1,IL-2 of rabbit with recurrent aphthous ulcers［J］.Heibei J TCM,2011,33(9):1381-1383.
[10]邹玉红,杨静,陈春华.复发性口腔溃疡患者血清中TNF-α、IL-2,6与免疫功能的相关性［J］.海南医学院学报,2015,21(9):1299-1301. ZOU YH,YANG J,CHEN CH.Correlation of TNF-α,IL-2,6 and immune function with recurrent oral ulcers［J］.Journal of Hinan Medical University,2015,21(9):1299-1301.
[11]KEEFE DM,SCHUBERT MM,ELTING LS,et al.Mucositis study section of the multinational association of supportive care in cancer and the international society for oral oncology.Updated clinical practice guidelines for the prevention and treatment of mucositis［J］.Cancer,2007,109(5):820-831.
[12]SONIS ST.Efficacy of palifermin(keratinocyte growth factor-1) in the amelioration of oral mucositis［J］.Core Evid,2010,4:199-205.
[13]谢靖,盛沛,李博,等.放射性口腔黏膜炎的发病机制及中西医防治研究进展［J］.南京中医药大学学报,2019,35(3):338-343. XIE J,SHENG P,LI B,et al.Pathogenesis of radiation-induced oral mucositis and prevention and treatment in TCM and modern medicine:A literature review［J］.J Nanjing Univ Tradit Chin Med,2019,35(3):338-343.
[14]杨秋燕,林月双,黄春叶.鼻咽癌放射性口腔黏膜反应的发病机理和防护进展［J］.护理实践与研究,2012,9(22):111-113. YANG QY,LIN YS,HUANG CY.Progress in pathogenesis and protection of radiation-induced oral mucosal reaction in nasopharyngeal carcinoma［J］.Nursing Practice and Research,2012,9(22):111-113.
[15]左念,梁靓.复方苦参注射液联合AP方案对非小细胞肺癌患者免疫功能及血清IL-6、IL-10、TNF-α水平的影响［J］.医学理论与实践,2021,34(24):4290-4291. ZUO N,LIANG L.Effect of compound Kushen injection combined with AP on immune function and serum IL-6,IL-10 and TNF-α levels in patients with non-small cell lung cancer［J］.J Med Theor ＆ Prac,2021,34(24):4290-4291.
[16]李妍琦.复方苦参注射液对晚期结直肠癌化疗者营养状况及免疫功能的影响［J］.中国肛肠病杂志,2021,41(11):14-15. LI YQ.Influence of Compound Kushen Injection in nutriture and immuno-function of patients with late colorectal cancer and undergoing chemotherapy［J］.Chin J Coloproctol,2021,41(11):14-15.
[17]王洪玲,段秀梅,海丽娜,等.复方苦参注射液总糖纯化方法及含量测定研究［J］.中国民族民间医药,2021,30(21):57-61. WANG HL,DUAN XM,HAI LN,et al.Research on purification method and determination of total sugar in Compound Kushen Injection［J］.Chinese Journal of Ethnomedicine and Ethnopharmacy,2021,30(21):57-61.
[18]项冰倩,何金波,高慧,等.右美托咪啶对肺缺血/再灌注损伤大鼠促炎介质IL-1β、TNF-α水平的影响及其机制［J］.中国应用生理学杂志,2017,33(05):415-419. XIANG BQ,HE JB,GAO H,et al.Effects of dexmedetomidine on the levels of proinflammatory mediators IL-1β and TNF-α in pulmonary ischemia/reperfusion injury rats and the mechanisms［J］.Chin J Appl Physiol,2017,33(05):415-419.
[19]郭俊兵,黄伟安,牛艳红,等.白细胞介素-6在大鼠放射性口腔黏膜炎中的表达及意义［J］.临床医学工程,2011,18(05):649-651. GUO JB,HUANG WA,NIU YH,et al.A study on the expression of IL-6 in rat's radiation-induced oral mucositis［J］.Clinical Medicine & Engineering,2011,18(05):649-651.
[20]SAND F L,THOMSEN SF.Efficacy and safety of TNF-alpha inhibitors in refractory primary complex aphthosis:A patient series and overview of the literature［J］.Journal of Dermatological Treatment,2013,24(6):444-446.
[21]GRUBER S,BOZSAKY E,ROITINGER E,et al.Early inflammatory changes in radiation-induced oral mucositis［J］.Strahlentherapie und Onkologie,2017,193(6):499-507.
[22]龙鑫,张智敏,谢刚,等.中药含漱液防治急性放射性口腔黏膜炎临床研究［J］.四川中医,2017,35(01):179-181. LONG X,ZHANG ZM,XIE G,et al.Clinical investigation of using Chinese medicine gargle therapy in the prevention and treatment of acute radiation-induced oral mucositis［J］.Journal of Sichuan of Traditional Chinese Medicine,2017,35(01):179-181.
[23]连利霞，孔存权， 雒建超，等.核黄素磷酸钠联合复方苦参注射液治疗鼻咽癌放射性口腔黏膜炎临床疗效观察［J］.现代肿瘤医学,2017,25(24):3957-3961. LIAN LX,KONG CQ,LUO JC,et al.Efficacy of riboflavin sodium phosphate combined with compound Kushen injection of radtioactive oral mucosal injury on nasopharyngeal carcinoma［J］.Modern Oncology,2017,25(24):3957-3961.

Memo

Memo:
国家中药标准化项目（编号：ZYBZH-C-JIN-43）；山西省重点研发计划重点项目（编号：201603D3113011）
Last Update: 2023-06-30