|Table of Contents|

Effect of PRDX6 silencing on proliferation,migration,invasion and radiosensitivity of esophageal cancer cells EC9706

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 15
Page:
2798-2802
Research Field:
Publishing date:

Info

Title:
Effect of PRDX6 silencing on proliferation,migration,invasion and radiosensitivity of esophageal cancer cells EC9706
Author(s):
SONG Jianyuan1; 2; 3; GUAN Hongdan1; 2; 3; ZHENG Rong1; 2; 3; GUAN Bingjie1 ; LIN Yuping1; 2; 3; WANG Bisi1; XU Benhua1; 2; 3
1.Department of Radiation Oncology,Fujian Medical University Union Hospital;2.Fujian Key Laboratory of Intelligent Imaging and Precision Radiotherapy for Tumors(Fujian Medical University);3.Clinical Research Center for Radiology and Radiotherapy of Fujian Province(Digestive,Hematological and Breast Malignancies),Fujian Fuzhou 350001,China.
Keywords:
PRDX6; EC9706; radiosensitivity
PACS:
R735.1
DOI:
10.3969/j.issn.1672-4992.2023.15.007
Abstract:
Objective：To investigate the effect of PRDX6 expression on the proliferation,migration,invasion and radiosensitivity of esophageal cancer cells EC9706.Methods:The expression of PRDX6 in esophageal cancer tissues was analyzed by GEPIA database."PRDX6-shRNA" plasmids which inhibit the expression of PRDX6 were transfected into EC9706 cells,and were record as sh-PRDX6 group.qRT-PCＲ and Western Blot were used to detect the transfection effect.CCK8 assay was used to detect the proliferation.Scratch assay was used to detect the migration.Transwell test was used to detect the invasion.Clone formation assay was used to detect the clone formation ability.Western Blot was used to detect the expressions of AKT and p-AKT proteins.Results：The expression of PRDX6 in esophageal carcinoma tissues was significantly higher than that of adjacent tissues.qRT-PCＲand Western Blot showed that "PRDX6-shRNA" plasmids effectively inhibited the expression of PRDX6.Compared with control group,cell proliferation ,migration and invasion ability of sh-PRDX6 group were significantly reduced,and the radiosensitivity was increased.The expression level of AKT protein in sh-PRDX6 group was not significantly changed,while the expression level of p-AKT protein significantly decreased.Conclusion:PRDX6 promotes the proliferation,migration,invasionand radioresistance of esophageal cancer cells EC9706.

References:

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Memo

Memo:
福建省自然科学基金面上项目（编号:2019J01149）
Last Update: 2023-06-30