|Table of Contents|

Construction of BNN6@PEG-HCuSNPs nanoparticles and verification of anti-colon cancer experimental effect

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 15
Page:
2765-2775
Research Field:
Publishing date:

Info

Title:
Construction of BNN6@PEG-HCuSNPs nanoparticles and verification of anti-colon cancer experimental effect
Author(s):
GONG Ming; CUI Ligang; ZHANG Jinxia; SUN Suhui; TANG Qingshuang
Institute of Medical Technology,Peking University Health Science Center,Beijing 100871,China.
Keywords:
colon cancer; BNN6@PEG-HCuSNPs nanoparticles; NIR-Ⅰ
PACS:
R735.3+5
DOI:
10.3969/j.issn.1672-4992.2023.15.003
Abstract:
Objective:To construct BNN6@PEG-HCuSNPs nanoparticles and observe its anti-colon cancer effect in vitro and in vivo.Methods:Construction of BNN6@PEG-HCuSNPs was performed by hydrothermal reaction.The photothermal,photodynamic and gas therapy effects of BNN6@PEG-HCuSNPs were observed in vitro.CT26 cells were selected as the research object.The uptake of BNN6@PEG-HCuSNPs by CT26 was observed under confocal microscope,and the effect of BNN6@PEG-HCuSNPs on ROS release from CT26 cells was detected by DCFH-DA fluorescence probe.DAF-FMDA fluorescent probe was used to detect the effect of BNN6@PEG-HCuSNPs on the release of NO from CT26 cells.BBoxiProbe fluorescent probe was used to detect the effect of BNN6@PEG-HCuSNPs on the release of ONOO- from CT26 cells.Calcein-AM and PI were used to detect the effect of BNN6@PEG-HCuSNPs on the survival of CT26 cells.Female Balb/c mice were taken as tumor models.When the tumor volume grew to about 150 mm3,the tumor-bearing mice were randomly divided into 6 groups,with 5 mice in each group:Control group,Laser group,PEG-HCuSNPs group,PEG-HCuSNPs+Laser group,BNN6@PEG-HCuSNPs group and BNN6@PEG-HCuSNPs+Laser group.During the treatment,saline or 20 mg/kg BNN6@PEG-HCuSNPs nanoparticles were injected into the corresponding groups through the tail vein on the 0 th and 3 rd days,respectively.The tumor volume and weight of each group were observed,and the cell structure was analyzed by HE staining.DNA fragments of cells were analyzed by TUNEL and Ki-67 antigen staining. Results:Transmission electron microscope,scanning electron microscope and 1H NMR showed that BNN6@PEG-HCuSNPs nanoparticles were successfully synthesized,and BNN6@PEG-HCuSNPs could be effectively endocytosed by CT26 cells.After irradiation with NIR-Ⅰ,BNN6@PEG-HCuSNPs could stably and effectively produce ROS and NO at the cellular level.The results of Calcein-AM and PI showed that irradiation of BNN6@PEG-HCuSNPs in NIR-Ⅰ can induce apoptosis of tumor cell CT26 and achieve therapeutic effect,and it had ONOO- obvious toxicity to HUVECs cells and CT26 cells.Photoacoustic imaging monitoring showed that BNN6@PEG-HCuSNPs could gather in the tumor site,and the photothermal/photodynamic/gas therapy produced by BNN6@PEG-HCuSNPs combined with NIR-Ⅰ was superior to other groups in tumor inhibition.The results of TUNEL showed that BNN6@PEG-HCuSNPs+Laser group caused a large number of tumor cells to apoptosis and showed more obvious green fluorescence than other groups.The results of Ki-67 antigen staining showed that compared with the control group,Laser group,PEG-HCuSNPs group,BNN6@PEG-HCuSNPs group and PEG-HCuSNPs+Laser group，the number of mesogenic nuclei in BNN6@PEG-HCuSNPs+Laser group decreased most obviously.Conclusion:BNN6@PEG-HCuSNPs nanoparticles have significant anti-colon cancer effects in vitro and in vivo.

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Last Update: 2023-06-30