|Table of Contents|

Expression of KIF14 in intrahepatic cholangiocarcinoma and its effects on proliferation and invasion of cholangiocarcinoma cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 14
Page:
2678-2682
Research Field:
Publishing date:

Info

Title:
Expression of KIF14 in intrahepatic cholangiocarcinoma and its effects on proliferation and invasion of cholangiocarcinoma cells
Author(s):
XUE Dong1; WU Junyi1; HE Xiaoyu2; LI Fanni2
1.Department of General Surgery;2.Department of Talent Highland,the First Affiliated Hospital of Xi'an Jiaotong University,Shaanxi Xi'an 710061,China．
Keywords:
intrahepatic cholangiocarcinoma; KIF14; proliferation; invasion; AKT signaling pathway
PACS:
R735.8
DOI:
10.3969/j.issn.1672-4992.2023.14.019
Abstract:
Objective:To investigate the expression of KIF14 in intrahepatic cholangiocarcinoma (ICC) and its effects on the biological behavior of cholangiocarcinoma cells.Methods:The mRNA expression level of KIF14 in ICC tissue and paracancerous tissue was detected by qRT-PCR.The protein expression of KIF14 in ICC tissue and paracancerous tissue was detected by immunohistochemistry,and the relationship between KIF14 expression and clinical pathological characteristics was analyzed.The siRNA technology was used to inhibit the expression of KIF14 in QBC939 cells and the inhibitory effect was detected.Western blotting assay was used to detect the effects of inhibiting the expression of KIF14 on AKT signaling pathway.We performed MTT and Transwell assay to detect the ability of proliferation and invasion after inhibiting the expression of KIF14 in QBC939 cells.Results:The result of qRT-PCR indicated that the mRNA expression level of KIF14 in ICC tissue was significantly higher than that in the paracancerous tissue.The result of immunohistochemistry staining indicated that the protein expression level of KIF14 in ICC tissue was higher than that in the paracancerous tissue.The expression level of KIF14 was related to the tumor size,lymph node metastasis and TNM stage.The siRNA technology could effectively inhibit the mRNA expression of KIF14 in QBC939 cells and decrease the phosphorylation level of AKT.The results of MTT assay and Transwell assay showed that inhibiting the expression of KIF14 can significantly reduce the ability of proliferation and invasion of QBC939 cells.Conclusion:KIF14 is highly expressed in ICC tissue and its high expression is associated with adverse clinical and pathological characteristics.KIF14 promotes the proliferation and invasion of cholangiocarcinoma cells through AKT signaling pathway.

References:

［1］DEOLIVEIRA ML,CUNNINGHAM SC,CAMERON JL,et al.Cholangiocarcinoma:thirty-one-year experience with 564 patients at a single institution［J］.Ann Surg,2007,245(5):755-762.
［2］RAZUMILAVA N,GORES GJ.Cholangiocarcinoma［J］.Lancet,2014,383(9935):2168-2179.
［3］高博,赵海鹰.肝内胆管癌的流行病学特征及危险因素研究进展［J］.现代肿瘤医学,2020,28(07):1214-1217. GAO B,ZHAO HY.Epidemiological characteristics and risk factors of intrahepatic cholangiocarcinoma［J］.Modern Oncology,2020,28(07):1214-1217.
［4］BENOIT MPMH,ASENJO AB,PAYDAR M,et al.Structural basis of mechano-chemical coupling by the mitotic kinesin KIF14［J］.Nat Commun,2021,12(1):3637.
［5］覃晓,李丽玲.Kif14调节肿瘤发生发展机制的研究进展［J］.广西医科大学学报,2019,36(12):2025-2029. QIN X,LI LL.Research progress of mechanism of Kif14 regulating tumorigenesis and development［J］.Journal of Guangxi Medical University,2019,36(12):2025-2029.
［6］YANG Z,LI C,YAN C,et al.KIF14 promotes tumor progression and metastasis and is an independent predictor of poor prognosis in human gastric cancer［J］.Biochim Biophys Acta Mol Basis Dis,2019,1865(1):181-192.
［7］NESKA DLUGOSZ I,BUCHHOLZ K,DURSLEWICZ J,et al.Prognostic impact and functional annotations of KIF11 and KIF14 expression in patients with colorectal cancer［J］.Int J MolSci,2021,22(18):9732.
［8］ZHANG Y,YUAN Y,LIANG P,et al.Overexpression of a novel candidate oncogene KIF14 correlates with tumor progression and poor prognosis in prostate cancer［J］.Oncotarget,2017,8(28):45459-45469.
［9］THERIAULT BL,BASAVARAJAPPA HD,LIM H,et al.Transcriptional and epigenetic regulation of KIF14 overexpression in ovarian cancer［J］.PLoS One,2014,9(3):e91540.
［10］BROWN KM,PARMAR AD,GELLER DA.Intrahepatic cholangiocarcinoma［ J］.Surg Oncol Clin N Am,2014,23(2):231-246.
［11］MANZIA TM,PARENTE A,LENCI I,et al.Moving forward in the treatment of cholangiocarcinoma［ J］.World J Gastrointest Oncol,2021,13(12):1939-1955.
［12］丛天豪,刘瑞宝.介入治疗联合其他治疗手段在肝内胆管癌治疗中的应用及进展［J］.现代肿瘤医学,2022,30(17):3221-3224. CONG TH,LIU RB.Application and progress of interventional therapy combined with other treatments in intrahepatic cholangiocarcinoma［J］.Modern Oncology,2022,30(17):3221-3224.
［13］SINGEL SM,CORNELIUS C,ZAGANOR E,et al.KIF14 promotes AKT phosphorylation and contributes to chemoresistance in triple-negative breast cancer［J］.Neoplasia,2014,16(3):247-256.
［14］WANG ZZ,YANG J,JIANG BH,et al.KIF14 promotes cell proliferation via activation of Akt and is directly targeted by miR-200c in colorectal cancer［J］.Int J Oncol,2018,53(5):1939-1952.
［15］AHMED SM,THERIAULT BL,UPPALAPATI M,et al.KIF14 negatively regulates Rap1a-Radil signaling during breast cancer progression［J］.J Cell Biol,2012,199(6):951-967.
［16］陈萍,厚玉瑾,吕燕,等.KIF14 mRNA在大肠癌中的表达及其临床意义［J］.宁夏医科大学学报,2016,38(05):512-514. CHEN P,HOU YJ,LV Y,et al.The expression of KIF14 mRNA in colorectal cancer and its clinical significance［J］.Journal of Ningxia Medical University,2016,38(05):512-514.
［17］CHEN P,FAN W,HOU Y,et al.Role of kinesin family member 14 in disease monitoring and prognosis in patients with gastrointestinal cancer［J］.Oncol Lett,2022,23(5):156.
［18］YANG T,ZHANG XB,ZHENG ZM.Suppression of KIF14 expression inhibits hepatocellular carcinoma progression and predicts favorable outcome［J］.Cancer Sci,2013,104(5):552-557.
［19］BASAVARAAPPA HD,CORSON TW.KIF14 as an oncogene in retinoblastoma:a target for novel therapeutics［J］.Future Med Chem,2012,4(17):2149-2152.
［20］SARAFIDIS M,LAMBROU GI,ZOUMPOURLIS V,et al.An integrated bioinformatics analysis towards the identification of diagnostic,prognostic,and predictive key biomarkers for urinary bladder cancer［J］.Cancers (Basel),2022,14(14):3358.

Memo

Memo:
National Natural Science Foundation of China(No.81803026);国家自然科学基金资助项目(编号：81803026)
Last Update: 1900-01-01