|Table of Contents|

Effect of KIF14 on biological characteristics of lung adenocarcinoma A549 cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 14
Page:
2638-2646
Research Field:
Publishing date:

Info

Title:
Effect of KIF14 on biological characteristics of lung adenocarcinoma A549 cells
Author(s):
LUAN Yanchao; PENG Haijun; YANG Qian; HAN Qingsong; WANG Mingzheng
Hebei Provincial Lung Cancer Prevention and Treatment Research Center,Hebei Chest Hospital,Hebei Provincial Key Laboratory of Lung Disease,Hebei Shijiazhuang 050001,China.
Keywords:
lung neoplasms; adenocarcinoma; proliferation; apoptosis; movement; KIF14
PACS:
R734.2
DOI:
10.3969/j.issn.1672-4992.2023.14.012
Abstract:
Objective:To investigate the expression of kinesin superfamily protein 14 (KIF14) in lung adenocarcinoma (LUAD) and its effect on the biological function of LUAD cells.Methods:The expression of KIF14 mRNA in LUAD was analyzed by TCGA and GEO databases.Kaplan-Meier method was used to analyze the relationship between KIF14 expression and the prognosis of patients.Single sample gene set enrichment analysis (ssGSEA) and TIMER database were used to explore the relationship between KIF14 expression level and tumor immune infiltration.The biological function of KIF14 in lung adenocarcinoma was annotated by functional enrichment analysis.Further in vitro experiments and immunohistochemistry were performed to verify the results.The expression of KIF14 in LUAD tissues and cell lines was detected by quantitative real-time PCR (qPCR) and Western blot.The KIF14 interference sequence was constructed and transfected into A549 cells,which were divided into NC group (transfected with KIF14 negative control sequence) and siKIF14 group (transfected with siRNA sequence targeting KIF14).The silencing effect of KIF14 in cells was detected by Western blot.CCK-8 and plate clone formation assay were used to detect the effect of siKIF14 on the proliferation of LUAD cells.TUNEL assay was used to detect the effect of siKIF14 on the apoptosis of LUAD cells.Transwell and scratch assays were used to detect the effect of siKIF14 on the invasion and migration of LUAD cells.Results:Bioinformatics database analysis showed that KIF14 mRNA was highly expressed in LUAD (P＜0.05),and the cumulative overall survival (OS) rate of patients with high KIF14 mRNA expression was poor (P＜0.05).Functional enrichment analysis showed that KIF14 was involved in cell division,cell cycle,DNA replication and other processes.ssGSEA and TIMER analysis showed a negative correlation between KIF14 expression and immune cell infiltration.KIF14 was highly expressed in LUAD tissues and A549 cells (P＜0.05).Silencing KIF14 expression inhibited the proliferation,invasion and migration of A549 cells,and induced cell apoptosis (P＜0.05).Conclusion:KIF14 may be a potential target in the diagnosis and treatment of lung adenocarcinoma.It may affect the occurrence and development of lung adenocarcinoma by regulating cell proliferation,migration and invasion.

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Memo

Memo:
河北省卫生健康委科研基金项目（编号：20231211）;河北省财政厅省直医疗卫生机构老年病防治项目资助（编号：361013）
Last Update: 1900-01-01